中华医学超声杂志

应用化学 ›› 1994, Vol. 0 ›› Issue (1): 92-95.

• 研究论文 • 上一篇    下一篇

固态磷脂相变行为的红外和拉曼光谱研究

王玮, 李来明, 席时权   

  1. 中国科学院长春应用化学研究所 长春 130022
  • 收稿日期:1993-03-20 修回日期:1993-09-27 出版日期:1994-02-10 发布日期:1994-02-10

Studies on Phase Transition Behavior of Anhydrous Phospholipid by lnfrared and Raman Spectroscopy

Wang Wei, Li Laiming, Xi Shiquan   

  1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022
  • Received:1993-03-20 Revised:1993-09-27 Published:1994-02-10 Online:1994-02-10

PDF

370

被引次数

1

摘要： 用红外和拉曼光谱方法在290～405K的温度范围内考察了无水二肉豆蔻酰磷脂酰乙醇胺的分子构象随温度的变化。结果表明,该磷脂在365K附近的熔化相变过程中,不仅酰链的构象和堆积发生了有序-无序转变,而且极性头部基团和接界区域基团也发生了结构重排;逆相变过程中,烷基链构象重新恢复了有序,但头部基团和甘油骨架构象没有恢复原始有序状态。

关键词: 二肉豆蔻酰磷脂酰乙醇胺, 分子构象, 红外光谱, 拉曼光谱

Abstract: The temperature dependence of the infrared and Raman spectra of anhydrous dimyristoylphosphatidylethanolamine (DMPE ) was investigated in the temperature range from 290 to 405K. The spectra of the C-H,C=Oand PO2- stretching mode regions reflect that a cooperative phase transition occurs at about 365K. During the transition a significant lateral expansion of the lipid bilayer is present and accompanied both by an increased number of gauche rotamers within the hydrocarbon chain region and by muItiple conformers in both the head group and glycerol backbone moieties. When the lipids are rapidly cooled,the acyl chain region reorders without a concomitant reordering of the head group and interface region.

Key words: dimyristoylphsphatidylethanolamine, molecular conformation, infrared spec-troscopy, Raman spectroscopy

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