中华医学超声杂志

应用化学 ›› 1993, Vol. 0 ›› Issue (3): 16-19.

• 研究论文 • 上一篇    下一篇

单一粒度法研究固定化酶内扩散限制的酶应动力学

邹文樵1, 冯仰婕1, 李冰2   

  1. 1. 华东化工学院化学系 2000237 上海;
    2. 中国科学院上海生物化学研究所 上海
  • 收稿日期:1992-05-03 修回日期:1992-12-08 出版日期:1993-06-10 发布日期:1993-06-10

STUDY ON THE KINETICS OF IMMOBILIZED ENZYME REACTION WITH INTERNAL DIFFUSION LIMITATION USING SINGLE SIZE PARTICLE

Zou Wenqiao1, Feng Yangjie1, Li Bing2   

  1. 1. East China Institute of Chemical Technology, 200237 Shanghai;
    2. Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai
  • Received:1992-05-03 Revised:1992-12-08 Published:1993-06-10 Online:1993-06-10

PDF

371

摘要： 本文提出了用一种粒度的固定化酶,利用在高底物浓度与低底物浓度时分别表现为零级反应与一级反应的特点,研究在连续式反应器中内扩散限制下酶反应动力学的方法。通过在棒条状异构化酶上葡萄糖转化为果糖的反应,测求了酶反应的本征参数以及一级反应速度常数、表观米氏常数、底物扩散系数、效率因子等动力学参数。

关键词: 固定化酶, 反应动力学

Abstract: A method was suggested for the study of immobilized enzyme kinetics with internal diffusion limitations in a fixed-bed reactor using single size immobilized enzyme. According to the characters of the reactions at enough high and low concentrations of substrates, corresponding to zero and first order reactions respectively, the intrinsic kinetics parameters and internal diffusion parameters for reaction of glucose isomerization were determined. The values of effectiveness factor were calculated and compared with that obtained in the experiment.

Key words: immobilized enzyme, reaction kinetics

网站版权 © 应用化学     本网站备案：吉ICP备12000082号