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多元曲线分辨-交替最小二乘法应用于延胡索药材指纹图谱中重叠峰分辨及延胡索乙素的含量测定

贾泽慧1,王春涛1,李华2*   

  1. (1.太原师范学院化学系 太原 030031；2.西北大学分析科学研究所/中药现代化研究中心 西安 710069)
  • 收稿日期:2012-04-27 修回日期:2012-06-25 出版日期:2013-03-10 发布日期:2013-03-10
  • 通讯作者: 李华，教授; Tel:029-88302635; Fax:029-88303527; E-mail:huali@nwu.edu.cn; 研究方向：生物分析化学和功能体系的化学信息学、过程分析及中药现代化
  • 基金资助:
    国家自然科学基金（20975081）山西省自然科学基金(2011011007-2)资助项目

The Applications of Multivariate Curve Resolution-alternating Least Squares in Resolving Overlapped Spectra of Corydalis yanhusuo Fingerprint and the Content Detection of Tetrahydropalmatine

JIA Zehui1, WANG Chuntao1, LI Hua2*   

  1. (1.Department of Chemistry,Taiyuan Normal College,Taiyuan 030031,China;
    2.Institute of Analytical Science/Research Center for Modernization of
    Chinese Herbal Medicines,Northwest University,Xi′an 710069,China)
  • Received:2012-04-27 Revised:2012-06-25 Published:2013-03-10 Online:2013-03-10

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1872

被引次数

2

摘要： 采用光谱相关色谱法对延胡索药材指纹图谱中的有效组分延胡索乙素峰进行确认；利用多元曲线分辨交替最小二乘法（MCR-ALS）对延胡索乙素重叠峰簇进行解析，得到延胡索乙素的真实色谱峰，其光谱还原率大于0.999，并对其进行准确定量，该方法可作为指纹图谱的辅助方法，为中药的真伪鉴定和质量客观评价提供可靠依据；最后讨论了药材的炮制对延胡索乙素含量的影响。

关键词: 延胡索药材, 中药指纹图谱, 光谱相关色谱, 多元曲线分辨-交替最小二乘法

Abstract: Spectral correlative chromatography was used to identify the peak of tetrahydropalmatine in Corydalis yanhusuo fingerprint. Moreover, multivariate curve resolution-alternating least squares(MCR-ALS) was used to resolve the overlapping peaks of tetrahydropalmatine and restore its true peak shape. More than 99.9% spectrum could be recovered and accurately quantitative results were obtained. The method can be used as an auxillary fingerprinting method to provide a reliable basis for the authenticity and quality assessment of Chinese medicines. The effect of the herbs-cooking on the content of tetrahydropalmatine was also discussed.

Key words: corydalis yanhusuo, fingerprint of traditional Chinese medicine, Spectral correlative chromatography, Multivariate curve resolution-alternating least squares

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