应用化学 ›› 2011, Vol. 28 ›› Issue (09): 1012-1016.DOI: 10.3724/SP.J.1095.2011.00375

• 研究论文 • 上一篇    下一篇

硫脲壳聚糖Co(Ⅱ)配合物的制备、抑菌活性及与血清白蛋白的作用

冯小强*,李小芳,杨声   

  1. (天水师范学院生命科学与化学学院 天水 741001)
  • 收稿日期:2010-06-28 修回日期:2010-09-12 出版日期:2011-09-10 发布日期:2011-09-10
  • 通讯作者: 冯小强,讲师; Tel/Fax:0938-8350899; E-mail:fengxiaoq04@lzu.cn; 研究方向:天然高分子生物活性
  • 基金资助:
    甘肃天水师范学院“青蓝”人才工程及物理无机化学重点学科基金资助项目(ZD0840)

Preparation, Antibacterial Activity of Thiourea-chitosan-Co(II) Complex and Interaction with Bovine Serium Albumin

FENG Xiaoqiang*, LI Xiaofang, YANG Sheng   

  1. (College of Life Science and Chemistry,Tianshui Normal University,Tianshui 741001)
  • Received:2010-06-28 Revised:2010-09-12 Published:2011-09-10 Online:2011-09-10

摘要:

合成了硫脲壳聚糖Co(Ⅱ)配合物,运用红外光谱、紫外光谱和差热热重分析对配合物进行了表征,研究了其对大肠杆菌和金黄色葡萄球菌的抑菌性能及与血清白蛋白(BSA)的相互作用。 结果表明,硫脲壳聚糖能与Co(Ⅱ)发生配位反应而生成硫脲壳聚糖-Co(Ⅱ)配合物,该配合物的抑菌性能较壳聚糖和硫脲壳聚糖有显著提高,并能有规律地猝灭BSA的内源荧光。

关键词: 硫脲壳聚糖Co(II)配合物, 抑菌性能, 血清白蛋白

Abstract:

Thiourea-chitosan-Co(Ⅱ) complex was prepared and characterized by FT-IR, UV-Vis absorbance spectra and TG-DTA methods, the antibacterial activities of thiourea-chitosan-Co(Ⅱ) complex against E.coli and St.aureus were studied in vitro. Moreover, the interaction between thiourea-chitosan-Co(Ⅱ) complex and bovine serum albumin(BSA) was also studied by ultraviolet and fluorescence spectroscopies. The results confirm that the complex is formed, and the antibacterial activities of thiourea-chitosan-Co(Ⅱ) complex is obviously enhanced comparing to its ligands and the complex can quench the intrinsic fluorescence of BSA.

Key words: Thiourea-Chitosan-Co(II) complex, Antibacterial activity, Bovine Serum Albumin

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