中华医学超声杂志

应用化学 ›› 2011, Vol. 28 ›› Issue (01): 107-112.DOI: 10.3724/SP.J.1095.2011.00195

• 研究论文 • 上一篇    下一篇

氟喹诺酮类抗生素与磷钨酸体系的共振瑞利散射光谱研究及其分析应用

李勤1,周尚2,杨季冬1,2*   

  1. (1.西南大学化学化工学院 重庆 400715；2.长江师范学院化学化工学院 重庆 408100)
  • 收稿日期:2010-04-06 修回日期:2010-07-06 出版日期:2011-01-10 发布日期:2011-01-10
  • 通讯作者: 杨季冬，教授; Tel:023-72790666; E-mail:flyjd6400@sina.com; 研究方向：分子光谱分析
  • 基金资助:
    重庆市教委科研资助项目(KJ081306)

Study on Resonance Rayleigh Scattering spectra of Phosphowolframic Acid and Fluoroquinolone Antibiotics System and theirs Analytical Application

LI Qin1, ZHOU Shang2, YANG Jidong1,2*   

  1. (1.School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715;
    2.School of Chemistry and Chemical Engineering,Yangtze Normal University,Chongqing 408100)
  • Received:2010-04-06 Revised:2010-07-06 Published:2011-01-10 Online:2011-01-10

PDF

1048

被引次数

32 | 3

摘要：

在5.0 mol/L的HCl缓冲介质中，磷钨酸（Pwa）与莫西沙星(MXFX)和加替沙星（GTF）等氟喹诺酮类抗生素(FLQs)相互作用形成摩尔比1∶1离子缔合物，导致体系的共振瑞利散射(RRS)显著增强并出现新的RRS光谱。 MXFX和GTF的反应产物具有相似的光谱特征，最大散射波长位于320 nm附近，且药物浓度与散射增强(ΔI)成正比，2种氟喹诺酮类药物的线性范围分别为0.025~6.0 mg/L（MXFX）和0.023~9.0 mg/L（GTF）。 据此可建立用于测定氟喹诺酮类药物的简捷快速灵敏的新方法，方法用于胶囊和人尿液中的FLQs测定并取得满意结果。 并对反应机理和RRS增强的原因进行了讨论。

关键词: 莫西沙星, 加替沙星, 磷钨酸, 共振瑞利散射光谱

Abstract:

In 5.0 mol/L HCl buffer solution, phosphowolframic acid(Pwa) could react with moxifloxacin(MXFX) and gatifloxacin(GTF) fluoroquinolone antibiotics(FLQs) to form 1∶1 ion-associated complexes respectively. The reactions resulted in great enhancement of resonance Rayleigh scattering(RRS) and new RRS bands appear. The reaction products of the two drugs had similar spectral characteristics, and the maximum RRS wavelength was located at 320 nm. The intensities of RRS are proportional to the concentration of antibiotics, the linearity ranges of two fluoroquinolone antibiotics are 0.025~6.0 mg/L(MXFX) and 0.023~9.0 mg/L(GTF), respectively. Therefore, a new method for determination of the drugs could be developed and applied to the determination of FLQs in capsules and human urine samples. The reaction mechanism and the reasons for RRS enhancement of the system were investigated.

Key words: Moxifloxacin, Gatifloxacin, Phosphowolframic Acid, Resonance Rayleigh Scattering Spectra

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