中华医学超声杂志

应用化学 ›› 2011, Vol. 28 ›› Issue (02): 214-218.DOI: 10.3724/SP.J.1095.2010.00220

• 研究论文 • 上一篇    下一篇

三维多孔碳纤维/聚乳酸/壳聚糖复合支架材料的体外细胞相容性评价

徐文峰1,廖晓玲2*   

  1. (1.重庆文理学院化学与环境工程学院 重庆 402160;
    2.重庆科技学院冶金与材料工程学院 重庆 401331)
  • 收稿日期:2010-04-15 修回日期:2010-06-22 出版日期:2011-02-10 发布日期:2011-02-10
  • 通讯作者: 廖晓玲，教授; E-mail:zxc_228@163.com; 电话:023-67023736; 研究方向：生物组织工程材料
  • 作者简介: 
    联系作者：廖晓玲，女，博士后，教授；E-mail:zxc_228@163.com；研究方向：复合材料
  • 基金资助:
    国家自然科学基金（30870609）重庆市教委自然科学基金(KJ081205，KJ091213)资助项目

Evaluation of Cell Compatibility for Porous 3D CF/PLA/CS Composites Scaffold in Vitro

XU Wen-Feng1, LIAO Xiao-Ling2*   

  1. (1.College of Chemistry and Environment Engineering,Chongqing University of Arts and Sciences,Chongqing 402160;
    2.College of Metallurgical and Materials Engineering,Chongqing University of Science and Technology,Chongqing 401331)
  • Received:2010-04-15 Revised:2010-06-22 Published:2011-02-10 Online:2011-02-10
  • About author:
    XU Wen-Feng 1 , LIAO Xiao-Ling 2*
    (1 College of Chemistry and Environment Engineering, Chongqing University of Arts and Sciences, Chongqing 402160, China
    2 College of Metallurgical and Materials Engineering, Chongqing University of Science and Technology Chongqing 401331, China)

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1739

被引次数

24 | 5

摘要：

利用溶液共混法以及冷冻干燥法制备了三维多孔碳纤维/聚乳酸/壳聚糖(CF/PLA/CS)复合生物支架材料，通过相差显微镜和扫描电子显微镜检测了鼠骨髓基质细胞（BMSCs）与该材料的生物相容性，得到了MTT生长曲线，评价了材料的毒性。 结果表明，以实验组材料的浸提液培养细胞，8 d后细胞开始连片生长，没有观察到细胞变形、坏死现象；在实验组材料上培养4 d后细胞的SEM图像显示，细胞形貌正常，并已开始向孔隙深部生长；MTT法绘制的增值曲线表明，培养4 d后实验组的细胞增殖速度高出空白对照组30%。 以上细胞形态学观察法和细胞增殖法评价结果表明，三维多孔 CF/PLA/CS复合材料没有细胞毒性，并对细胞有良好的粘附、增殖能力，有望成为骨修复材料。

关键词: 三维, CF/PLA/CS复合材料, 生物体外测试, 细胞相容性

Abstract:

The porous carbon-fiber/poly(lactic acid)/chitosan(CF/PLA/CS) composites scaffold was preparated by using solution blending and freeze-drying technique, and the biocompatibility between the composites and bone marrow stromal cells(BMSCs) was estimated by using inverted phase contrast microscope and scanning electron microscope(SEM). The curve of cell growth was obtained by using MTT assay, and the cell cytotoxicity were also assessed. The results showed that the cultured cells did not appear the deformation or death in the leach liquor and grew densely after 8 days. The SEM images showed that the cell morphology was normal, and the cell started to grow along the deep pore on the composites after 4 days. The MTT curve of cell growth indicated that cell proliferation rate of the experimental group was 30% higher than the blank test group after 4 days. According to the morphological observation and cell proliferation evaluation, it indicated the three-dimensional porous CF/PLA/CS composites do not have cell toxicity, and have excellent adhesion and proliferation of bone cells, suggesting that they may be promising bone repairing materials.

Key words: 3D, CF/PLA/CS Composites, In vitro, Cell compatibility

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