应用化学 ›› 2017, Vol. 34 ›› Issue (6): 723-728.DOI: 10.11944/j.issn.1000-0518.2017.06.160376

• 研究论文 • 上一篇    下一篇

高效液相色谱-飞行时间质谱联用法分析人参及人参皂苷与山楂配伍过程中的水解行为

郑飞ab,王微a,于珊珊a,戴雨霖a,刘尚a,文连奎b*(),越皓a*()   

  1. a长春中医药大学,吉林省人参科学研究院 长春 130117
    b吉林农业大学食品科学与工程学院 长春 130118
  • 收稿日期:2016-09-20 接受日期:2016-12-28 出版日期:2017-05-31 发布日期:2017-05-31
  • 通讯作者: 文连奎,越皓
  • 基金资助:
    国家自然科学基金(3140040275)资助

Hydrolysis Behaviors of Combined Dectotion of Panax ginseng and Ginsenosides with Hawthorn by HPLC-Q-TOF MS/MS

ZHENG Feiab,WANG Weia,YU Shanshana,DAI Yulina,LIU Shanga,Wen Liankuib*(),YUE Haoa*()   

  1. a Jilin Ginseng Academy,Changchun University of Chinese Medicine,Changchun 130117,China
    b College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China
  • Received:2016-09-20 Accepted:2016-12-28 Published:2017-05-31 Online:2017-05-31
  • Contact: Wen Liankui,YUE Hao
  • Supported by:
    Supported by the National Natural Science Foundation of China(No.3140040275)

摘要:

利用高效液相色谱-飞行时间质谱联用的方法,分别对人参配伍山楂前后人参皂苷的变化进行分析,同时对人参皂苷Re、Rg1、Rb1、Rd与山楂配伍的水解规律进行系统研究,并与单独煎煮液、仿山楂配伍pH值煎煮液的水解产物进行比较,结果发现人参与山楂配伍后人参皂苷Rg1、Rb1含量明显减少,而人参皂苷Re、Rd、Rg2、Rg3、F2、Rh1含量明显增加,其中人参皂苷Re与山楂配伍后水解产物为人参皂苷20(R)-Rg2、20(S)-Rg2,仿山楂配伍pH值水解产物为人参皂苷20(R)-Rg2、20(S)-Rg2、Rg4、Rg6;人参皂苷Rg1与山楂配伍后水解产物为20(S)-Rh1、20(R)-Rh1,仿山楂pH值水解产物为20(S)-Rh1、20(R)-Rh1、Rh4、Rk3;人参皂苷Rb1与山楂配伍后水解产物为Rd、20(S)-Rg3,仿山楂pH值水解产物为F2、20(S)-Rg3;人参皂苷Rd与山楂配伍后水解产物为F2、20(S)-Rg3、20(R)-Rg3,仿山楂pH值水解产物为20(S)-Rg3、20(R)-Rg3。 研究表明,不同人参皂苷和山楂配伍后与仿山楂pH值的水解产物并不相同,人参与山楂配伍改变了人参皂苷成分的种类及含量。 本研究为临床方剂中人参与山楂配伍后成分的变化提供物质基础数据。

关键词: 高效液相色谱-飞行时间质谱, 人参皂苷, 配伍, 水解

Abstract:

High performance liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry(HPLC-Q-TOF MS/MS) were used for the comparative analysis of the content of ginsenosides in the decoction of Panax ginseng with hawthorn and hydrolysis of ginsenosides in compatibility of ginsenosides Re, Rg1, Rb1, Rd with hawthorn, single decoction and simulated pH value decoction. The results show that the contents of ginsenosides Rg1 and Rb1 decrease, while the contents of ginsenosides Re, Rd, Rg2, Rg3, F2, Rh1 increase in ginseng-hawthorn combined decoction. Ginsenosides 20(R)-Rg2, 20(S)-Rg2 are the hydrolysates when ginsenoside Re and hawthorn are boiled together, while others are ginsenosides 20(R)-Rg2, 20(S)-Rg2, Rg4 and Rg6 when ginsenoside Re is boiled in the same pH value without hawthorn. In addition, the hydrolysates are ginsenosides 20(S)-Rh1 and 20(R)-Rh1 in ginsenoside Rg1-hawthorn combined decoction, but ginsenosides 20(S)-Rh1, 20(R)-Rh1, Rh4, Rk3 are determined in the simulated pH value decoction. Ginsenosides Rd, 20(S)-Rg3 are the major hydrolysates in co-decoction of ginsenoside Rb1 and hawthorn, while ginsenosides F2, 20(S)-Rg3 are the major hydrolysates from the simulated pH value decoction. Ginsenosides F2, 20(R)-Rg3, 20(S)-Rg3 are hydrolysates in ginsenoside Rd-hawthorn combined decoction, but ginsenosides 20(R)-Rg3, 20(S)-Rg3 are hydrolysates in the simulated pH value decoction. The hydrolysis of ginsenosides is different in the processes of co-decoction with hawthorn and the simulated pH value decoction, which may be the basis of chemical composition in combination of Panax ginseng and hawthorn. It provides the material bases for combination of Panax ginseng and hawthorn in prescriptions of traditional Chinese medicine.

Key words: HPLC-Q-TOF MS/MS, ginsenoside, combination, hydrolysis