Effects of Photodynamic Therapy Mediated by Coumarin Derivative Against Drug-Resistant Bacteria

doi:10.19894/j.issn.1000-0518.240233

Chinese Journal of Applied Chemistry ›› 2024, Vol. 41 ›› Issue (11): 1639-1647.DOI: 10.19894/j.issn.1000-0518.240233

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Effects of Photodynamic Therapy Mediated by Coumarin Derivative Against Drug-Resistant Bacteria

Hong-Shuang QIN¹, Shu-Han CHEN¹, Xu-Wei HAN⁴, Yan-Xiang GUO¹, Ru-Nan MIN⁴, Tao LIU³(), Chuan-Qi ZHAO²()

^1.Department of Biological and Food Engineering，Lyuliang University，Lvliang 033001，China
^2.Laboratory of Chemical Biology，Changchun Institute of Applied Chemistry，Chinese Academy of Sciences，Changchun 130022，China
^3.Department of Chemical and Materials Engineering，Lyuliang University，Lvliang 033001，China
^4.School of Chemistry and Chemical Engineering，Shanxi University，Taiyuan 030006，China

Received:2024-09-08 Accepted:2024-10-20 Published:2024-11-01 Online:2024-12-04
Contact: Tao LIU,Chuan-Qi ZHAO
About author:zhaocq@ciac.ac.cn；
liutao@llu.edu.cn
Supported by:
the National Natural Science Foundation of China(22122704);the Jilin Province Science and Technology Development Plan Project(20210101130JC);the Basic Research Project of Shanxi Province(202303021211336);the Shanxi Province Graduate Education Reform Project(2022YJJG302);the Key Research Program of Lvliang City(2022SHFZ39)

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Abstract

Abstract:

Blue light （BL） mediated photodynamic therapy （PDT） can not only effectively kill drug-resistant bacteria， but also reduce the damage to deep tissues， providing a new opportunity for the treatment of drug-resistant bacterial infections. However， the blue light photosensitizers used in PDT against bacteria are very limited， and it is urgent to develop new blue light photosensitizers to overcome the growing problem of drug resistance of bacteria. Coumarin derivatives exhibit many biological activities， such as anti-inflammatory， antibiosis and anti-tumor， as well as good photosensitivity and stability. Herein， the bactericidal effects of coumarin derivative CDOCA-mediated PDT against methicillin-resistant Staphylococcus aureus （MRSA） were studied. The results showed that 32 μg/mL CDOCA irradiated with blue light （445 nm， 0.5 W/cm²） for 5 min could completely inhibit the vitality of planktic bacteria. Moreover， CDOCA （48 μg/mL） combined with blue light （0.5 W/cm²， 6 min） could fully eliminate MRSA wrapped in biofilm and degrade biofilm. More importantly， the combined treatment of CDOCA （1 mg/kg） and BL （0.5 W/cm²， 6 min） for 8 days effectively eradicated the biofilm on the skin wounds of mice， reduced inflammation and promoted wound healing. CDOCA-mediated PDT provided in this study is expected to be used in the treatments of drug-resistant bacterial infections.

Key words: Drug-resistant bacterium, Photodynamic therapy, Coumarin derivative, Biofilm, Blue light

CLC Number:

O643.1

Hong-Shuang QIN, Shu-Han CHEN, Xu-Wei HAN, Yan-Xiang GUO, Ru-Nan MIN, Tao LIU, Chuan-Qi ZHAO. Effects of Photodynamic Therapy Mediated by Coumarin Derivative Against Drug-Resistant Bacteria[J]. Chinese Journal of Applied Chemistry, 2024, 41(11): 1639-1647.

Figures/Tables 5

Fig.1 Synthesis method of CDOCA

Fig.2 Chemical structure （A） and UV-Vis spectrum （B） of CDOCA， as well as live/dead stain images of MRSA treated with CDOCA+BL （C）， scale bar=20 μm

Fig.3 Membrane potentials （A）， ATP levels （B） and ROS production （C） in MRSA treated with CDOCA+BL

Fig.4 Quantification of MRSA in biofilms （A）， quantization of biofilms （B）， microscopic images of biofilms stained by crystal violet （scale bar=100 μm）（C） and 3D confocal images of biofilms （D） treated with CDOCA+BL

Fig.5 Schematic illustration of the experimental procedure for eliminating biofilms from infected mice （A）； Digital photographs of the wounds on mice （scale bar=1 mm）（B）， number of the surviving bacteria in the wound tissues （scale bar=20 mm）（C， D） and micrographs of skin wound tissues with H&E staining （scale bar=50 μm）（E） after treatments of CDOCA+BL

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Effects of Photodynamic Therapy Mediated by Coumarin Derivative Against Drug-Resistant Bacteria

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