Preparation and Application of Auramine O Imprinted Sensor Based on Nanomaterials Modification

doi:10.19894/j.issn.1000-0518.220092

Chinese Journal of Applied Chemistry ›› 2022, Vol. 39 ›› Issue (12): 1880-1890.DOI: 10.19894/j.issn.1000-0518.220092

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Preparation and Application of Auramine O Imprinted Sensor Based on Nanomaterials Modification

Li QIN¹, Xiao-Ting YOU¹, Lu-Hua TANG¹, Jian-Wen LI², Yin ZHANG², Wen-Hui GAO¹(), Jun-Hua HAN¹()

^1.College of Food and Biology，Hebei University of Science and Technology，Shijiazhuang 050000，China
^2.Shijiazhuang Food and Drug Inspection Center，Shijiazhuang 050011，China

Received:2022-03-26 Accepted:2022-08-06 Published:2022-12-01 Online:2022-12-13
Contact: Wen-Hui GAO,Jun-Hua HAN
About author:teach2003@126. com
wenhuigao@126. com
Supported by:
the Key Research and Development Program Project of Hebei Province(19275505D);the Science and Technology Research and Development Plan Project of Shijiazhuang(211170183A)

1. .jpg(7770KB)

Abstract

Abstract:

Auramine O may be illegally used as a food colorant because of its cheapness and easy to enhance color， causing great harm to human health. Therefore， it is of great significance to establish a sensitive and rapid method for the determination of auramine O in food. In the experiment， reduced graphene oxide and gold nanoparticles sol are used to modify working electrodes， a polymer film is formed by cyclic voltammetry on the surface of the modified electrode with auramine O as template molecule， o-aminophenol and o-phenylenediamine as composite functional monomers. The template molecules are eluted using constant potential of -0.1 V in methanol-0.5 mol/L NaOH aqueous solution （1∶1， V/V）， the molecularly imprinted electrochemical sensor （MIECS） is prepared successfully for detection of auramine O. Electrochemical analysis method is used to optimize the ratio of template molecules to composite functional monomers. The polymer solution pH and cylinder number， the morphology and chemical structure of the membrane are characterized by scanning electron microscope and infrared spectrometer. The imprinting effect and analysis performance of the sensor are investigated. The results show that the sensor has specific adsorption to auramine O. Under the optimal conditions， the auramine O has a good linear relationship in the concentration range of 1.0×10^-9~3.0×10^-5 mol/L， the detection limit is 3.3×10^-10 mol/L， and the average recoveries are between 91.16% and 101.61% with the relative standard deviations （RSDs） of 2.79%. The sensor has good stability and reproducibility， and is suitable for sensitive and rapid detection of auramine O in food samples.

Key words: Auramine O, Composite functional monomer, Molecularly imprinted electrochemical sensor, Electrochemical analysis, Rapid detection

CLC Number:

O657.1

Li QIN, Xiao-Ting YOU, Lu-Hua TANG, Jian-Wen LI, Yin ZHANG, Wen-Hui GAO, Jun-Hua HAN. Preparation and Application of Auramine O Imprinted Sensor Based on Nanomaterials Modification[J]. Chinese Journal of Applied Chemistry, 2022, 39(12): 1880-1890.

Figures/Tables 8

Fig.1 Optimizing the dosage of trisodium citrate by UV-visible spectroscopy （A） and electrochemical method （B）， and optimization of the ratio between RGO modifier and AuNPs by electrochemical method （C）

Fig.2 CV curves of the eluted electrode in different eluents （A） and optimization of auramine O elution time （B）a. Methanol?0.5 mol/L NaOH aqueous solution (volume ratio 1∶1); b. Acetonitrile?0.5 mol/L NaOH aqueous solution (volume ratio 1∶1); c. Methanol?0.2 mol/L H2SO4 aqueous solution (volume ratio 2∶1)

Fig.3 SEM images of AuNPs/RGO/GCE （A）， MIP with auramine O （B）， MIP after removal of auramine O （C） and EDS spectrum of AuNPs/RGO/GCE （D）

Fig.4 FT-IR spectra of auramine O （a）， MIP with auramine O （b）， MIP after auramine O removal （c） and NIP （d）

Fig.5 CV curves of the different electrodes in the solutiona. MGCE； b. MIP/MGCE after auramine O removal； c. bare GCE； d. MIP/MGCE after readsorption auramine O； e. MIP/MGCE with auramine O

Fig.6 Selectivity of the imprinted sensora. 0.1 mmol/L auramine O； b. 1 mmol/L methyl red； c. 1 mmol/L dimethyl yellow； d. 1 mmol/L rhodamine B； e. a and 10 times methyl red； f. a and 10 times dimethyl yellow； g. a and 10 times rhodamine B

Table 1 Recoveries and precision in spiked samples （n=5）

样品 Sample	豆皮 Bean curd			腐竹 Bean curd stick			辣椒面 Chili powder			黄鱼 Yellow croaker
不同添加水平 Added levels/（nmol·L^-1）	1	30	500	1	30	500	1	30	500	1	30	500
测定次数Number of measurements	回收率 Recovery/%
1	92.37	95.46	101.54	90.35	98.25	102.34	93.52	97.18	100.24	89.85	96.38	100.73
2	93.54	98.23	99.71	91.86	97.08	101.57	88.43	92.92	101.45	88.74	97.92	101.45
3	90.88	97.31	100.89	93.70	97.16	99.83	94.01	93.83	99.19	93.57	95.01	100.58
4	91.72	95.85	102.27	89.22	96.34	100.16	90.68	94.45	99.53	94.69	95.23	99.62
5	90.03	97.79	103.63	93.59	95.62	101.92	89.17	93.79	101.67	93.21	97.64	102.91
平均回收率AR/%	91.71	96.93	101.61	91.74	96.89	101.16	91.16	94.43	100.42	92.01	96.44	101.06
相对标准偏差RSD/%	1.47	1.25	1.45	2.15	1.02	1.09	2.76	1.72	1.11	2.79	1.39	1.21

Table 2 The comparison of the method and other methods for the determination of auramine O

方法 Methods	线性范围 Linear range/（mol·L^-1）	检出限 Detection limit/（μg·L^-1）	文献 Ref.
HPLC-MS/MS	—	0.75	DB35/T 897-2009
HPLC	1.6×10^-7~3.3×10^-4	16	［6］
LC-MS/MS	1.6×10^-9~3.3×10^-8	0.067	［27］
SERS	8.2×10^-9~6.6×10^-8	0.82	［28］
UHPLC-MS	8.2×10^-9~3.3×10^-7	0.049	［29］
MIECS	1.0×10^-9~3.0×10^-5	0.1	This work

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Preparation and Application of Auramine O Imprinted Sensor Based on Nanomaterials Modification

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