Recent Progress of Library Construction for Next-generation Sequencing

doi:10.19894/j.issn.1000-0518.200158

Chinese Journal of Applied Chemistry ›› 2021, Vol. 38 ›› Issue (1): 11-23.DOI: 10.19894/j.issn.1000-0518.200158

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Recent Progress of Library Construction for Next-generation Sequencing

LI Lin, QIAN Si-Hua, LYU Tian-Qi, WANG Yu-Hui^*, ZHENG Jian-Ping^*

Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering NIMTE, Chinese Academy of Sciences, Ningbo 315201, China

Received:2020-05-25 Revised:2020-12-10 Published:2021-01-01 Online:2021-02-01
Supported by:
Natural Science Foundation of Zhejiang Province(No.LY20B050003), Ningbo 3315 Innovation Team(No.2019A-14-C) and the Major Science and Technology Projects in Ningbo(No.2016C50009)

Abstract

Abstract: High-throughput next-generation sequencing (NGS) is a revolutionary technology in gene sequencing. It is widely used in various solutions in biomedical-relevant fields. Library preparation with high quality and low-cost is the key for NGS. With the development of such sequencing platforms, different library preparation technologies have been built. However, these technologies also possess their own strengths and weaknesses. In this review, we first summarized various library preparation methods for NGS, then discussed the complete process of library preparation in single cell sequencing. We hope that this review can help researchers on the optimal selection of library preparation strategies and also offer suggestions for the development of new NGS library preparation technologies, especially in the exploitation of homebred kits.

Key words: Next-generation sequencing, Single cell sequencing, Library preparation

CLC Number:

O661

LI Lin, QIAN Si-Hua, LYU Tian-Qi, WANG Yu-Hui, ZHENG Jian-Ping. Recent Progress of Library Construction for Next-generation Sequencing[J]. Chinese Journal of Applied Chemistry, 2021, 38(1): 11-23.

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Recent Progress of Library Construction for Next-generation Sequencing

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