Modification of Ordered Macroporous Silica by a Functional Polymer Layer and Immobilization of Glucoamylase on the Macropore Walls

doi:10.11944/j.issn.1000-0518.2018.11.170371

Chinese Journal of Applied Chemistry ›› 2018, Vol. 35 ›› Issue (11): 1309-1316.DOI: 10.11944/j.issn.1000-0518.2018.11.170371

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Modification of Ordered Macroporous Silica by a Functional Polymer Layer and Immobilization of Glucoamylase on the Macropore Walls

YE Lifang,WU Quanzhou()

School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China

Received:2017-10-18 Accepted:2018-01-22 Published:2018-10-31 Online:2018-10-31
Contact: WU Quanzhou
Supported by:
Supported by the National Natural Science Foundation of China(No.81303199), the Guangdong Province Natural Science Foundation of China(No.2017A030313675), the Youth Elite Project of GUCM(No.AFD015151Z1450)

Abstract

Abstract:

This paper presented a facile method to the modification of the three-dimensionally ordered macroporous(3DOM) SiO₂ hybrids with a carboxyl functionalized polymer layer on the macropore walls. Acrylic acid and glycerol 1,3-diglycerolate diacrylate were copolymerized to form the polymer layer in the macropores. Raman spectra, scanning electron microscopy(SEM) and BET measurements show that the prepared 3DOM SiO₂@polymer composites(SiO₂-COOH) have an uniform interconnected macroporous structure and the macropore walls are covered by a nanoscaled compact polymer layer. Moreover, the 3DOM SiO₂-COOH has an improved mechanical strength. The 3DOM SiO₂-COOH was further used as the support to immobilize glucoamylase. The results show that the immobilized enzyme homogeneously distributes in the 3DOM materials. The optimum pH of immobilized and free enzymes is both at 5, and the optimal reaction temperature is at 55 ℃. Michaelis constants of the immobilized enzyme and free enzyme are 3.78 g/L and 3.97 g/L, respectively. The immobilized enzyme presents higher thermal, pH, storage stabilities and higher reusability compared with the free enzyme. The results indicate that 3DOM SiO₂-COOH could be a novel support for the immobilization of enzymes.

Key words: ordered macropore, surface modification, composite, immobilized enzyme

YE Lifang,WU Quanzhou. Modification of Ordered Macroporous Silica by a Functional Polymer Layer and Immobilization of Glucoamylase on the Macropore Walls[J]. Chinese Journal of Applied Chemistry, 2018, 35(11): 1309-1316.

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Modification of Ordered Macroporous Silica by a Functional Polymer Layer and Immobilization of Glucoamylase on the Macropore Walls

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