Abstract:

A preconcentration column packed with home-made sulfonated glycidyl methacrylate(GMA)-grafted-polytetrafluoroethylene(PTFE) polymer(PTFE-g-GMA-SO3H) fiber, coupled with high-performance liquid chromatography(HPLC) and flow injection, fiber was developed for trace melamine determination in the samples. The on-line determination method using the fiber as adsorbent for trace melamine in two milk sample was demonstrated. The conditions for preconcentration and elution of melamine were optimized. In addition, analytical performance of trace melamine determination was acquired in this article. The enrichment factor for melamine was 300 and the detection limits was 1.13×10-2 mg/L. The relative standard derivation was 7.6%（n=9） when the melamine concentration was 0.2 mg/L, and the recovery of samples was 98% and 102.5%, respectively.

Key words: sulfonated glycidylmethacrylate grafted polytetrafluoroethylene fiber, on line solid extration, melamine