Abstract:

Water-soluble polysaccharide B(BRP-B) in Blumea riparia(BI.)DC. was extracted and purified by using membrane separation, discoloration on diethylaminoethyl(DEAE)-cellulose, and sephadex column chromatography of G10 and G50. Results from high-performance gel permeation chromatography(HPGPC) showed that the obtained BRP-B has a narrow-distributed molecular mass, with the number average molecular mass and mass average molecular mass as 2654 and 2716, respectively. The drug activity test based on plasma recalcification as the index showed that BRP-B with the optimal concentration of 6.25×10-2 g/L had the strongest procoagulant activity, the coagulation time was 248.52 s, and the inhibition rate of coagulation time was 22.42%. In particularly, BRP-B with concentration of 1.67×10-2, 1.97×10-2 and 2.85×10-2 g/L could significantly enhance the frequency of uterus muscle.

Key words: Blumea riparia (BI．)DC, polysaccharide, separation, purification, hemostatic activity