Abstract:

A fluorescent method for the synchronous determination of 1-naphylamine and 2-naphylamine was developed. In order to avoid the interference from the two isomers, the synchronous fluorescence and first-order-derivative synchronous fluorescence spectra of the two isomers of naphylamine were studied, and the zero-crossing method for reading spectral intensities was adopted. At pH=7.8(phosphate buffered water solution) and Δλ=120 nm, the synchronous fluorescence peaks were at 323 nm and 290 nm for 1-napylamine and 2-naphylamine, respectively. The first order derivative synchronous fluorescence intensities varied linearly with 1-naphylamine and 2-naphylamine concentrations in a range of 4.0×10-7~2.0×10-5 mol/L. The relative standard deviations(RSDs) of them were all below 5%. The limits of detection were 4.0×10-8 and 2.9×10-8 mol/L for 1-naphylamine and 2-naphylamine, respectively. The method was applied to the simultaneous determination of the two naphylamine isomers with satisfactory results.

Key words: Naphylamine, Synchronous-derivative fluorescence spectrum