Abstract:

Abstract： A  new method was proposed for the determination of moroxydine hydrochloride with  sodium nitroprusside by spectrophotometry. Colored  N-nitrosamine product was  formed by moroxydine hydrochloride and sodium nitroprusside in alkaline solution. The liner regression equation is A = 0.02292 + 0.01213c (10^-6 g/mL) with a correlation coefficient of 0.9998, and ε was 2.5×10³ L/(mol•cm) at  508 nm. Beer's law was well obeyed in a range of  moroxydine hydrochloride concentration of 0.38×10^-6  ~ 3.0×10^-4 g/mL,  the  limit of detection (3σ/k) was 0.25×10^-6 g/mL, the RSD was 0.21%. The method was used for  determination of moroxydine hydrochloride in pharmaceutical samples and human serum with satisfactory results.

Key words: sodium nitroprusside, moroxydine hydrochloride, acetone, spectrophotometry