Spectrophotometric Determination of Ampicillin Sodium Using Ninhydrin as Chromogenic Agents

doi:10.3724/SP.J.1095.2012.00160

Chinese Journal of Applied Chemistry

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Spectrophotometric Determination of Ampicillin Sodium Using Ninhydrin as Chromogenic Agents

ZHANG Yaqiu

(Pharmaceutical College of Liaoning Medical University,Jinzhou 121001)

Received:2011-04-19 Revised:2011-07-05 Published:2012-03-10 Online:2012-03-10

Abstract

Abstract: The reaction between ampicillin sodium and ninhydrin was investigated by spectrophotometry. A simple and rapid method for the determination of ampicillin sodium has been optimized. The apparent molar absorptivity of complex is 3.72×103 L/(mol·cm) at 564 nm. Beer′s law could be followed in the range of 10~90 mg/L of ampicillin sodium with correlation coefficient of 0.9981. The relative standard deviation is 1.5%(n=6) and the recovery was over 99.0%.

Key words: spectrophotometry, ampicillin sodium, ninhydrin

CLC Number:

O657.3

ZHANG Yaqiu. Spectrophotometric Determination of Ampicillin Sodium Using Ninhydrin as Chromogenic Agents[J]. Chinese Journal of Applied Chemistry, DOI: 10.3724/SP.J.1095.2012.00160.

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Spectrophotometric Determination of Ampicillin Sodium Using Ninhydrin as Chromogenic Agents

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