Abstract:

A purine nucleotide derivative 6-chloro-2-aminopurine nucleoside triphosphate was chemically synthesized through four steps and was characterized with 1H NMR, 31P NMR, 13C NMR, EA and IR. Its ultraviolet and fluorescence properties at various pH values were studied. One pKa value for the compound was determined by the curves of UV absorption dependency on pH, which was 1.44. Hydrolysis of the nucleotide derivative in the catalysis of potato apyrase was studied. The competion of the ATP analogue with ATP for potato apyrase凷 active site was proved to be a mixed prohibition mechanism.