Abstract:

he extraction of glutathione (GSH) from distillate of bread yeast with reversed micelles made up of phosphoric acid dioctyl ester (P204) and n-octanol was researched. The effect factors on Extraction rate of GSH were examined. The optimum conditions were confirmed in the orthogonal tests that extraction time is 15 min; pH value in aqueous phase is 2.0; concentration of P204 is 0.16 mol•L-1 and concentration of K+ is 0.1 mol•L-1. The extraction rate of GSH is 33.1% and the twi-extraction rate got 49.7% in the optimum conditions.

Key words: Bread yeast, Glutathione (GSH), Extraction with reversed micelles, reversed micelles of P204 / octanol