Rapid Resolution Liquid Chromatography with Tandem Mass Spectrometry on Pharmacokinetics of Type 2 Diabetic  Rats with Oral Administration of Sijunzitang Decoration

doi:10.19894/j.issn.1000-0518.200362

Chinese Journal of Applied Chemistry ›› 2021, Vol. 38 ›› Issue (3): 315-322.DOI: 10.19894/j.issn.1000-0518.200362

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Rapid Resolution Liquid Chromatography with Tandem Mass Spectrometry on Pharmacokinetics of Type 2 Diabetic Rats with Oral Administration of Sijunzitang Decoration

GAO Song-Hong¹, YANG Di², LI Zhong-Qi³, ZHENG Fei², JEON You-Jin^4*, DAI Yu-Lin^2,4*

¹Changchun Medical College, Changchun 130117, China
²Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China
³Jilin International Travel Health Care Center, Changchun 130062, China
⁴Department of Marine Life Science, Jeju National University, Jeju 63243, Republic of Korea

Received:2020-12-03 Accepted:2021-01-24 Published:2021-03-01 Online:2021-05-01
Supported by:
National Key Research and Development Program of China (No.2017YFC1702105) and the Science and Technology Development Program of Jilin Province (Nos.20180311019YY, 20180311039YY, 20180311031YY)

Abstract

Abstract: To study the pharmacokinetics of Sijunzi Decoction in type 2 diabetes rats. The water extract of Sijunzi Decoction was given to normal rats and type 2 diabetic rats by gavage at 18 g/kg. The urine and feces were collected at different time points, and the contents of its components and metabolites were detected. The pharmacokinetic parameters of normal rats and type 2 diabetic rats were compared by using rapid resolution liquid chromatography/tandem mass spectrometry (RRLC/MS). Ginsenoside Rc, glycyrrhiza and their metabolites were characterized by fast high resolution liquid chromatography-mass spectrometry. In Sijunzi Decoction, the cumulative excretion rate of ginsenoside Rc and glycyrrhizin in type 2 diabetic rats is higher than that in normal rats. Most ginsenosides are excreted in urine and metabolized into rare ginsenosides in feces. Most of glycyrrhizin is converted to glycyrrhetinic acid in urine and feces. There are significant differences in pharmacokinetic parameters of ginsenoside Rc and glycyrrhizin between type 2 diabetic rats and normal rats. The pharmacokinetic mechanism and metabolic pathway of Sijunzi Decoction in vivo were summarized.

Key words: Sijunzi Decoration, Pharmacokinetics, Type 2 diabetes, Rapid resolution liquid chromatography/tandem mass spectrometry

CLC Number:

O629

GAO Song-Hong, YANG Di, LI Zhong-Qi, ZHENG Fei, JEON You-Jin, DAI Yu-Lin. Rapid Resolution Liquid Chromatography with Tandem Mass Spectrometry on Pharmacokinetics of Type 2 Diabetic Rats with Oral Administration of Sijunzitang Decoration[J]. Chinese Journal of Applied Chemistry, 2021, 38(3): 315-322.

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Rapid Resolution Liquid Chromatography with Tandem Mass Spectrometry on Pharmacokinetics of Type 2 Diabetic Rats with Oral Administration of Sijunzitang Decoration

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