Synthesis and Application of a Coumarin Fluorescently Labeled Peptide

doi:10.3724/SP.J.1095.2014.40090

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Synthesis and Application of a Coumarin Fluorescently Labeled Peptide

LI Yan¹, LIU Xiaoyan^2,4, ZHANG Shun^2,4, LIU Ting^3,4, LI Yongjun^2,4, LAN Yanyu^2,4, WANG Aimin^2,4, WANG Yonglin^3,4, HE Bin^3,4*

(1.College of Basic Medical Science;
2.Engineering Research Center for the Development and Application of
Ethnic Medicine and TCM(Ministry of Education;
3.Guizhou Provincial Key Laboratory of Pharmaceutics,Guiyang Medical College;
4.College of Pharmacy,Guiyang Medical University,Guiyang 550004,China)

Received:2014-03-24 Revised:2014-06-13 Published:2014-11-08 Online:2014-11-08

Abstract

Abstract: Sirtuin is a type of nicotinamide adenine dinucleotide(NAD) dependent histone deacetylase, which has seven members. It is a potential therapeutic target for many diseases. However, the present fluorescent screening method is limited to SIRT1~SIRT3. Therefore, based on the discovery of SIRT5′s novel enzymatic activity, we designed and synthesized the fluorescence labeled peptide for SIRT5, ISGASE(SuK)-AMC; and by the validations of LC-MS and fluorescence detection, the fluorescence labeled peptide can be used as a tool to perform the screening based on the enzymatic activity of SIRT5.

Key words: histone deacetylase, Sirtuin, SIRT5, fluorescently labeled peptides

CLC Number:

O654

LI Yan1, LIU Xiaoyan2,4, ZHANG Shun2,4, LIU Ting3,4, LI Yongjun2,4, LAN Yanyu2,4, WANG Aimin2,4, WANG Yonglin3,4, HE Bin3,4*. Synthesis and Application of a Coumarin Fluorescently Labeled Peptide[J]. Chinese Journal of Applied Chemistry, DOI: 10.3724/SP.J.1095.2014.40090.

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Synthesis and Application of a Coumarin Fluorescently Labeled Peptide

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