Chinese Journal of Applied Chemistry ›› 2009, Vol. 26 ›› Issue (12): 1461-1465.

• Full Papers • Previous Articles     Next Articles

EB Fluorescence Probe on the Interaction between Dinuclear Macrocyclic Polyamine Zn(Ⅱ) Complex and DNA

LI Shuo1,2, ZHOU Cheng-He1, CHEN Jia-Xuan2, XIANG Qing-Xiang2*   

  1. (1.Department of Chemistry and Chemical Engneering,Southwest Univercity,Chongqing;
    2.Department of Chemistry and Life Science,Leshan Teachers College,Leshan 614004)
  • Received:2008-12-17 Revised:2009-04-19 Published:2009-12-10 Online:2009-12-10


A novel dinuclear macrocyclic polyamine Zn(Ⅱ) complex was synthesized from 1,4,7,10-tetraazacyclododecane, the structures of the compounds were characterized by 1H NMR, MS and elemental analysis. The interactions between calf thymus(CT) DNA and these compounds were investigated by fluorometric detection. The fluorescence intensity of the DNA/EB system was decreased to 47.3% of that of the initial system at c(complex 6)/c(DNA)=0.51, which indicates that the dinuclear complex was inserted into the CT DNA. The order of fluorescence quenching values of the DNA/EB systems by these ompounds:dinuclear macrocyclic polyamine Zn(Ⅱ) complexd>dinuclear macrocyclic polyamine ligand>CyclenZn(Ⅱ) complex>Cyclen.

Key words: dinuclear macrocyclic polyamine,Zn(Ⅱ) complex,synthesis,CT DNA,fluorescence

CLC Number: