Abstract: An electropolymerization method of m-phenylene diamine membrane, polyphenylene diamine(PMPD) on the surface of graphite electrode is described. To identify the quality of the membrane, a rapid and sensitive method has been established based on the reaction of a horse-radish peroxidase(HRP) labeled PMPD with orthophenylene diamine. An anti-HBsAg and an antiMIgG immunoelectrodes were prepared for detecting hepatitis B surface antigen(HBsAg) and mouse immunoglobin G(MIgG). The optimum membrane preparation conditions are polymerization: 0.12 mol/L MPD solution in 1.2 mol/L H₂SO₄ for 20 min under 2.5 V, activation: with 3% glutiraldehyde for 4 h. The prepared HRP labeled electrode and immunoelectrodes possessed good repetition. Catibration graphs were linear for HBsAg in ranges from 0.132 g/mL and 0.120 g/mL for MIgG/mL.

Key words: immunosensor, chemically modified electrode, poly-m-phenylene diamine