Abstract: Sodium bis(2-ethylhexyl polyoxyalkylene) sulfosuccinates of different polymerization numbers were synthesized. The activities of lipase in the reversed micelles formed by synthetic surfactants and normal AOT in isooctane have been compared in the process of catalytic hydrolysis of olive oil. The results showed the activity of lipase in sodium bis(2-ethylhexyl polyoxyethylene) sulfosuccinate(n=2 3)/isooctane reversed micelles was 2-fold higher than that in the normal AOT/isooctane reversed micelles.

Key words: sodium bis(2-ethylhexyl polyoxyalkylene) sulfosuccinate, surface activity, reversed micellar enzymatic reaction, olive oil hydrolysis