Potentiometric Determination of α-Fetoprotein (AFP) Using a Non-labeled Antibody Immunosensor

Chinese Journal of Applied Chemistry ›› 1998, Vol. 0 ›› Issue (1): 47-49.

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Potentiometric Determination of α-Fetoprotein (AFP) Using a Non-labeled Antibody Immunosensor

Peng Tuzhi, Zhu Fangmeng, Yang Liju, He Qiaohong, Cheng Qiong

Department of Chemistry, Hangzhou University, Hangzhou 310028

Received:1997-04-09 Revised:1997-09-10 Published:1998-02-10 Online:1998-02-10

Abstract

Abstract: The anti-AFP antibody is immobilized on the surface of PVC-BSA(bovine serumalbumin) membrane, which has been activated by NH₂OH·HCl solution.The immunosen-sor consists of such a membrane with added AFP and Ag-AgCl internal electrode.It showedlinear response over concentration range of 10-300μg/L and potential difference(△E) of 15~20 mV for 300μg/L of AFP.Other common antigens in human serum do not interfere withAFP's determination.

Key words: α-fetoprotein, potentiometry, immunosensor

Peng Tuzhi, Zhu Fangmeng, Yang Liju, He Qiaohong, Cheng Qiong. Potentiometric Determination of α-Fetoprotein (AFP) Using a Non-labeled Antibody Immunosensor[J]. Chinese Journal of Applied Chemistry, 1998, 0(1): 47-49.

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Potentiometric Determination of α-Fetoprotein (AFP) Using a Non-labeled Antibody Immunosensor

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