Preparation of Gold Nanorod Self-Assembled Flexible Films and Ratiometric Surface-Enhanced Raman Scattering Detection of Bacteria

doi:10.19894/j.issn.1000-0518.240391

Chinese Journal of Applied Chemistry ›› 2025, Vol. 42 ›› Issue (2): 182-191.DOI: 10.19894/j.issn.1000-0518.240391

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Preparation of Gold Nanorod Self-Assembled Flexible Films and Ratiometric Surface-Enhanced Raman Scattering Detection of Bacteria

Lu-Lu TIAN¹, Song-Tao HU², Xu-Zhou QU², Lin WANG¹(), Biao DONG²()

^1.Department of Oral Implantology，School and Hospital of Stomatology，Jilin University，Changchun 130012，China
^2.State Key Laboratory on Integrated Optoelectronics，College of Electronic Science and Engineering，Jilin University，Changchun 130012，China

Received:2024-11-28 Accepted:2025-01-08 Published:2025-02-01 Online:2025-03-14
Contact: Lin WANG,Biao DONG
About author:dongb@jlu.edu.cn
wanglin1982@jlu.edu.cn；
Supported by:
the National Natural Science Foundation of China （No.?52272080） and the Natural Science Foundation of Jilin Province(20220402005GH)

Abstract

Abstract:

Bacteria are key factors in causing infections， and quantitative detection for bacteria is crucial for clinical monitoring and treatment guidance. Gold nanorods （Au NRs） were self-assembled into dense metallic films using a liquid-liquid interface method and successfully transferred onto an ultrathin thermoplastic polyurethane surface， forming a uniform and stable flexible surface-enhanced Raman scattering （SERS） active substrate. This substrate was modified with 4-mercaptophenylboronic acid （MPBA） to provide a reference signal for bacterial fixation. On this substrate， the detection limit for MPBA molecules can reach 1×10^-9 mol/L. A gold-silver core-shell structured SERS tag was prepared， using 5，5'-dithiobis （2-nitrobenzoic acid）（DTNB） in the interlayer as the target signal. The signal of SERS tags can be enhanced by about 54 times by the prepared substrate. By combining SERS tags with nucleic acid aptamers， specific recognition of Escherichia coli （E.coli） was achieved. By analyzing the ratio of Raman characteristic peak intensities between the target signal and the reference signal （I₁₃₃₇/I₁₀₇₈）， a good linear relationship was observed between bacterial concentration in the range of 1×10² to 1×10⁷ CFU/mL and I₁₃₃₇/I₁₀₇₈ in water and saliva samples， with a coefficient of determination （R² =0.97126）. It was superior to analyzing the target signal alone （R² =0.87046）， demonstrating that ratiometric analysis provided a better fit. This strategy shows potential application value in bacterial detection.

Key words: Liquid-liquid interface method, Gold nanorods, Surface-enhanced Raman scattering, 4-Mercaptophenylboronic acid, Ratiometric analysis

CLC Number:

O657.3

Lu-Lu TIAN, Song-Tao HU, Xu-Zhou QU, Lin WANG, Biao DONG. Preparation of Gold Nanorod Self-Assembled Flexible Films and Ratiometric Surface-Enhanced Raman Scattering Detection of Bacteria[J]. Chinese Journal of Applied Chemistry, 2025, 42(2): 182-191.

Figures/Tables 5

Fig.1 （A） Schematic diagram of the assembly and structure of the Au Film；（B） The structure of Au@DTNB@Ag@Apt；（C） The detection mechanism of Au Film@MPBA and Au@DTNB@Ag@Apt to E.coli by the sandwich recognition method

Fig.2 （A） TEM image and （B） length and diameter statistical data of Au NRs；（C） Zeta potential of Au NRs and Au@MPBA；（D） TEM image，（E） physical dimensions， and （F） texture of Au Film；（G） UV-Vis spectra of Au NRs and Au Film；（H） Raman spectra of Au Film and Au Film@MPBA；（I） SERS activity of Au Film@MPBA with different layers

Fig.3 （A） SERS results and （B） linear fitting curve of the Au Film with different concentrations of MPBA；（C） SERS activity at different sites on Au Film@MPBA；（D） SERS activity of different batches of Au Film@MPBA；（E） SERS results collected from the front and back of Au Film@MPBA；（F） SERS performance of Au Film@MPBA after storage for 0 d， 7 d， 3 months， and 6 months

Fig.4 （A） TEM image of Au@DTNB@Ag； Elemental distribution of （B） Au and （C） Ag of Au@DTNB@Ag；（D） UV-Vis spectra of Au@DTNB and Au@DTNB@Ag；（E） SERS activity of Au@DTNB and Au@DTNB@Ag；（F） Raman enhancement of blank TPU and Au Film@MPBA to Au@DTNB@Ag；（G） Optical microscopy image of Au Film@MPBA capturing E.coli

Fig.5 （A） SERS results of sandwich-like substrates at different E.coli concentrations （0~1×107 CFU/mL） in water； The relationship （B） between the logarithmic concentration of E.coli and I1337/I1078 or （C） between the logarithmic concentration of E.coli and I1337 in water；（D） SERS results at different E.coli concentrations （0~1×107 CFU/mL） in saliva；（E） The relationship between the logarithmic concentration of E.coli and I1337/I1078 in saliva；（F） I1337/I1078 on substrates incubated with mixed bacteria （E.coli， S.aureus， F.nucleatum and P.gingivalis）， E.coli， S.aureus， F.nucleatum or P.gingivalis

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Preparation of Gold Nanorod Self-Assembled Flexible Films and Ratiometric Surface-Enhanced Raman Scattering Detection of Bacteria

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