Construction of a Photoelectrochemical Biosensor Based on ZnS-Modified Electrode for the Determination of L-Cysteine

doi:10.19894/j.issn.1000-0518.240222

Chinese Journal of Applied Chemistry ›› 2025, Vol. 42 ›› Issue (3): 321-329.DOI: 10.19894/j.issn.1000-0518.240222

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Construction of a Photoelectrochemical Biosensor Based on ZnS-Modified Electrode for the Determination of L-Cysteine

Jie-Xia CHEN, Zhuo-Er LIU, Chang-Ning LU, Ke-Xin YANG, Zi-Yan CHEN, Yan WEI()

School of Pharmacy，Wannan Medical College，Wuhu 241002，China

Received:2024-07-21 Accepted:2025-01-21 Published:2025-03-01 Online:2025-04-11
Contact: Yan WEI
About author:yanwei@wnmc.edu.cn
Supported by:
the Research Fund for Major Natural Science Research Projects in Universities of Anhui Province(2023AH040250);the University Natural Science Research Project of Anhui Province(KJ2021A0842);the Undergraduate Research Grants Programme(WK2023XS43)

Abstract

Abstract:

L-Cysteine（L-Cys） levels in serum are correlated with many diseases， and their highly sensitive detection is of great significance. A novel strategy for selective detection of L-Cys based on photoelectrochemical catalytic oxidation was developed utilizing the electrochemical deposition of ZnS nanomodified electrodes. Under light irradiation， ZnS nanoparticle underwent a process of electron-hole pair formation. The thiol groups （—SH） on L-Cys in the solution provided electrons to occupy the holes， and the photoinduced electrons on the conduction band were injected into the ITO electrode to form an anodic photocurrent. Meanwhile， the L-Cys in the solution underwent a reduction process， whereby electrons were transferred to form cystine containing disulfide bridge （—S—S—）， which then underwent a photoelectrochemical catalytic oxidation process. The photoelectric conversion efficiency was improved. The photocurrent of the sensor based on the photochemical catalytic oxidation mechanism increased linearly in the concentration range of 2~50 μmol/L， and obtained the detection limit of 0.67 μmol/L. This method was applied to determine the content of L-Cys in human serum， and the recovery rates were in the range of 97.6% to 104.6%.

Key words: Photoelectrochemical analysis, ZnS, L-Cysteine

CLC Number:

O657.1

Jie-Xia CHEN, Zhuo-Er LIU, Chang-Ning LU, Ke-Xin YANG, Zi-Yan CHEN, Yan WEI. Construction of a Photoelectrochemical Biosensor Based on ZnS-Modified Electrode for the Determination of L-Cysteine[J]. Chinese Journal of Applied Chemistry, 2025, 42(3): 321-329.

Figures/Tables 7

References 35

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Method	Linear range/（mol·L^-1）	Detection limit/（mol·L^-1）	Ref.
Capillary electrophoresis	1.0×10^-6~5.0×10^-4	8.76×10^-7	［6］
Fluorescence	0~0.135×10^-3	5.7×10^-6	［8］
Electrogenerated chemiluminescence	10.0×10^-6~100×10^-6	8.8×10^-6	［9］
Colorimetric method	99.9×10^-6~998.7×10^-6	1.0×10^-6	［10］
Liquid chromatography-ultraviolet method	80×10^-6~230×10^-6	4×10^-6	［11］
Electrochemistry	0.5×10^-6~10×10^-6	0.14×10^-6	［12］
Photoelectrochemical method CdS/TiO₂	5.0×10^-9~1.0×10^-6	1.3×10^-9	［22］
Photoelectrochemical method CuO/Cu₂O/C	0.2×10^-6~50×10^-6	0.12×10^-6	［23］
Photoelectrochemical method ZnS	2×10^-6~50×10^-6	0.67×10^-6	This work

Method	Linear range/（mol·L^-1）	Detection limit/（mol·L^-1）	Ref.
Capillary electrophoresis	1.0×10^-6~5.0×10^-4	8.76×10^-7	［6］
Fluorescence	0~0.135×10^-3	5.7×10^-6	［8］
Electrogenerated chemiluminescence	10.0×10^-6~100×10^-6	8.8×10^-6	［9］
Colorimetric method	99.9×10^-6~998.7×10^-6	1.0×10^-6	［10］
Liquid chromatography-ultraviolet method	80×10^-6~230×10^-6	4×10^-6	［11］
Electrochemistry	0.5×10^-6~10×10^-6	0.14×10^-6	［12］
Photoelectrochemical method CdS/TiO₂	5.0×10^-9~1.0×10^-6	1.3×10^-9	［22］
Photoelectrochemical method CuO/Cu₂O/C	0.2×10^-6~50×10^-6	0.12×10^-6	［23］
Photoelectrochemical method ZnS	2×10^-6~50×10^-6	0.67×10^-6	This work

Sample	Added/（μmol·L^-1）	Found/（μmol·L^-1）	Recovery/%	RSD/%（n=3）
1	0	10.17	-	3.2
2	5	15.16	99.8	4.3
3	10	19.93	97.6	3.5
4	20	31.09	104.6	2.7

Sample	Added/（μmol·L^-1）	Found/（μmol·L^-1）	Recovery/%	RSD/%（n=3）
1	0	10.17	-	3.2
2	5	15.16	99.8	4.3
3	10	19.93	97.6	3.5
4	20	31.09	104.6	2.7

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Construction of a Photoelectrochemical Biosensor Based on ZnS-Modified Electrode for the Determination of L-Cysteine

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