Small Molecule Recognition Based Photoelectrochemical Sensor for Histidine Detection

doi:10.19894/j.issn.1000-0518.230035

Chinese Journal of Applied Chemistry ›› 2024, Vol. 41 ›› Issue (1): 128-136.DOI: 10.19894/j.issn.1000-0518.230035

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Small Molecule Recognition Based Photoelectrochemical Sensor for Histidine Detection

Cui-Cui YANG, Xiao-Xue YE()

College of Chemistry and Chemical Engineering，College of Health Science and Engineering，Hubei University，Wuhan 430072，China

Received:2023-02-22 Accepted:2023-05-18 Published:2024-01-01 Online:2024-01-30
Contact: Xiao-Xue YE
About author:yexxchem@hubu.edu.cn
Supported by:
the National Natural Science Foundation of China(22276047)

1. .pdf(327KB)

Abstract

Abstract:

Abnormal change of histidine （His） level in serum may be associated with various diseases， making the detection of His clinically significant. Photoelectrochemical （PEC） sensing has the potential to be a promising clinical detection method due to its high sensitivity， simple operation， low cost， and ease of miniaturization. However， to date， PEC sensing for His detection has not been reported due to the lack of suitable recognition mechanisms. In this study， we construct a PEC sensing interface using a small molecule probe as the recognition unit， and the selectivity of the sensor is improved through a specific reaction between the probe and His. The developed （PEC） sensor has good selectivity， stability， and reproducibility， which can be used for the quantitative detection of His in biological samples.

Key words: Photoelectrochemical sensing, Histidine, Small molecular probes

CLC Number:

O657

Cui-Cui YANG, Xiao-Xue YE. Small Molecule Recognition Based Photoelectrochemical Sensor for Histidine Detection[J]. Chinese Journal of Applied Chemistry, 2024, 41(1): 128-136.

Figures/Tables 11

Fig.1 （A） Schematic of small molecule probe recognition based photoelectrochemical sensor for His detection；（B） Reaction mechanism of NCH-Cu2+ with His

Fig.2 Synthetic route of the organic small molecule NCH

Fig.3 XRD pattern of CdTe QDs

Fig.4 （A） UV-Vis absorption spectra of CdTe QDs， NCH-Cu2+ and NCH-Cu2+-His；（B） UV-Vis absorption spectra of NCH-Cu2+ in the presence of the varying amount of His（0， 10， 20， 40， 70 and 100 μmol/L）

Fig.5 （A） Photocurrent response and （B） EIS diagram of PEC sensor

Fig.6 Effects of the concentration of AA （A）， CdTe QDs （B）， NCH-Cu2+ （C） and the reaction time （D） on the photocurrent response

Fig.7 The photocurrent responses （A） and calibration curve （B） of PEC sensor for detecting different concentrations of His

Fig.8 Selectivity of the PEC sensor

Fig.9 Stability （A） and reproducibility （B） of the constructed PEC sensor

Fig.10 Detection of histidine in rat serum by PEC sensor

Table 1 Determination of His in diluted rat serum by the standard recovery method

Sample	Added ρ/（μg·mL^-1）	Detected ρ/（μg·mL^-1）	Recovery/%	RSD St/%
1	10	19.13	95.90	3.78
2	15	25.02	103.20	3.12
3	20	29.74	101.00	4.07

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Small Molecule Recognition Based Photoelectrochemical Sensor for Histidine Detection

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