Preparation and Properties of Mussel-Inspired Modified Gelatin-Based Hemostatic Sponge

doi:10.19894/j.issn.1000-0518.210050

Chinese Journal of Applied Chemistry ›› 2022, Vol. 39 ›› Issue (02): 247-257.DOI: 10.19894/j.issn.1000-0518.210050

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Preparation and Properties of Mussel-Inspired Modified Gelatin-Based Hemostatic Sponge

Zhu-Ying ZHANG, De-Yan DU, Jia-Hua ZHOU, Dong-Jian SHI(), Ming-Qing CHEN

The Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China

Received:2021-01-28 Accepted:2021-05-17 Published:2022-02-10 Online:2022-02-09
Contact: Dong-Jian SHI
Supported by:
Postgraduate Research& Practice Innovation Program of Jiangnan University(JNKY19_047);Natural Science Foundation of Jiangsu Province(BK20181349)

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Abstract

Abstract:

Uncontrollable hemorrhage induced by war or surgery is closely related to pain and high risk of death. Therefore, a timely and effective hemostatic material is highly required for life-threatening traumatic bleeding. However, current hemostatic materials still have various defects and side effects such as slow hemostasis and secondary infection. Herein, gelatin-dopamine-lysine (GDL) and oxidized dextran (ODE) were used to prepare sponge-like hemostasis materials through lyophilization. The results show that GDL/ODE is able to integrate with biological tissue and form physical barriers, which stops bleeding after 60 s of application. GDL/ODE composites show good cyto/hemo-compatibility and highly porous microscopic morphology and water absorption ratio. These results suggest that the developed GDL/ODE sponge is a promising multifunctional hemostasis material for biomedical fields.

Key words: Functional modification, Gelatin derivative sponge, Porous structure, Hemostasis materials

CLC Number:

O636

Zhu-Ying ZHANG, De-Yan DU, Jia-Hua ZHOU, Dong-Jian SHI, Ming-Qing CHEN. Preparation and Properties of Mussel-Inspired Modified Gelatin-Based Hemostatic Sponge[J]. Chinese Journal of Applied Chemistry, 2022, 39(02): 247-257.

Figures/Tables 7

Fig.1 Schematic illustration of fabrication of sponges

Fig.2 （A）Schematic illustration of synthesis of GDL.（B）UV absorption and（C）1H NMR spectra of GDL-H（a），GDL-L（b）and Gel（c）.（D）Schematic illustration of the synthesis of ODE and（E）1H NMR spectra of dextran（a）and ODE（b）

Table 1 Dopamine and lysine contents of GDL with different feeding ratios

样品	明胶质量	多巴胺的物质的量	赖氨酸的物质的量	多巴胺含量	赖氨酸含量
Sample	m（Gelatin）／g	n（DA）／mmol	n（Lys）／mmol	DA content／（μmol·g^－1） ^a	Lys content／（μmol·g^－1） ^b
GDL-L	5.00	19.50	19.50	30.18	49.80
GDL-H	5.00	39.00	39.00	36.56	57.61

Fig.3 （A）SEM images of internal morphologies of hemostasis sponges.（B）Porosities of hemostasis sponges.（C）Water absorptions of hemostasis sponges.（D）Time consumed for water droplets spreading out on the materials.（E）Snapshots of the droplets during the contact angle test of GDL-H4／ODE1（compared to Gel／ODE with the same ratio，*P<0.1，**P<0.01，***P<0.001；compared to pure GDL or Gel，+P<0.05，++P<0.01，+++P<0.001）

Fig.4 （A）Photograph of adhesive bridging between skin substrate and sponge.（B）Adhesion strength of hemostasis sponges.（C）Compression modulus of hemostasis sponges.（compared to Gel／ODE with the same ratio，*P<0.1；compared to pure GDL or Gel，+P<0.05，++P<0.01）

Fig.5 Cell compatibility of hemostasis sponges.（A）Cell viability of hemostatic sponges after cultured of 1，2 and 3 d.（B）Fluorescence images for L929 cells after cultured of 1 d.（n＝5，mean±SD，compared to Control，*P<0.05，**P<0.01，***P<0.001）

Fig.6 Hemostatic performance of materials.（A）The state of blood after coagulation in the blood clotting time test.（B）Blood clotting time，（C）blood clotting index，and（D）adhesion of erythrocyte.（E）hemolysis rate.（F）Photographs of the livers of mice during the liver injury model of hemostasis.（G）SEM images of cells adhesion on the sponges.（*P<0.05，**P<0.01，***P<0.001）

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Preparation and Properties of Mussel-Inspired Modified Gelatin-Based Hemostatic Sponge

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