咔咯铁(Ⅲ)配合物与DNA的作用及其抗肿瘤活性

doi:10.11944/j.issn.1000-0518.2019.12.190066

摘要/Abstract

摘要：

本文合成了10-(4-吩噻嗪苯基)-5,15-二(五氟苯基)铁咔咯配合物(2-Fe)。在H₂O₂存在下,配合物2-Fe能有效抑制人肺癌细胞(A549)增殖。流式细胞术检测结果显示在H₂O₂和2-Fe共同作用下,A549细胞生长被阻滞在S期和G2期,且有63.76%发生细胞凋亡。 Hoechst-33342细胞核染色实验观察到A549细胞核呈典型的凋亡形态学变化,同时细胞线粒体膜电势发生明显下降。 2-Fe与小牛胸腺DNA(ct-DNA)以外部模式相结合,且能有效催化氧化断裂DNA,此过程中涉及的活性氧物种可能是单线态氧。

关键词: 咔咯, 吩噻嗪, DNA, 细胞毒性, 铁, 细胞凋亡

Abstract:

10-(4-Phenothiazine)-5,15-bis(pentafluorophenyl)corrolatoiron (2-Fe) was synthesized. Complex 2-Fe displays high cytotoxic activity against human lung adenocarcinoma (A549) cell lines. Flow cytometry detected 2-Fe arrested the cell cycle in the S and G2 phase in the presence of H₂O₂ and apoptotic rate was 63.76%. Hoechst-33342 staining showed considerable morphological changes in nuclear chromatin and mitochondrial transmembrane potential dropped respectively. Complex 2-Fe could interact with DNA via an outside binding mode and trigger ¹O₂ generation.

Key words: corrole, phenothiazine, DNA, cytotoxicity, iron, apoptosis

史蕾, 杨文聪, 沈淇, 尹伟, 霍朝晖, 司利平, 刘海洋. 咔咯铁(Ⅲ)配合物与DNA的作用及其抗肿瘤活性[J]. 应用化学, 2019, 36(12): 1376-1386.

SHI Lei, YANG Wencong, SHEN Qi, YIN Wei, HUO Zhaohui, SI Liping, LIU Haiyang. Iron Corrole Complexes: DNA-Binding and Anti-tumor Activity[J]. Chinese Journal of Applied Chemistry, 2019, 36(12): 1376-1386.

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