基于电化学安培法的中药降血糖活性成分筛选

doi:10.11944/j.issn.1000-0518.2018.07.170312

摘要/Abstract

摘要：

为建立一种简单高效的中药活性成分的提取分离、定性鉴定、含量测定以及降血糖活性研究的新方法,采用绿色无毒的水提酸沉法提取了黄芩的活性成分—黄芩苷,通过薄层色谱法做定性鉴定和紫外分光光度法测定其含量,构建一个高效的电化学葡萄糖传感器,并建立一种基于电化学安培法的降血糖活性研究新方法。以临床降糖药阿卡波糖论证了电化学安培法检测葡萄糖淀粉酶抑制活性的可靠性和稳定性,并成功应用于黄芩苷的降血糖活性研究。结果表明,黄芩苷具有较好的葡萄糖淀粉酶抑制活性,其半数抑制浓度(IC₅₀)为0.056 g/L。本研究为从天然药物中提取分离降血糖活性成分提供了一种新颖的研究方法。

关键词: 黄芩苷, 葡萄糖淀粉酶, 电化学法, 抑制活性

Abstract:

A novel method for the extraction, isolation, identification and determination of the active component of Chinese medicine and its inhibitory activity on glucoamylase in a simple and efficient way was developed. The active ingredient baicalin was extracted from Scutellaria baicalensis using green nontoxic water-extracting and acid-depositing method. It was identified qualitatively by thin layer chromatography and the content was determined by ultraviolet spectrophotometry. An effective electrochemical glucose sensor was fabricated and a new method of characterizing hypoglycemic activity was developed based on electrochemical amperometric detection. Acarbose(clinical antidiabetic drug) was further used to demonstrate the reliability and stability of determining the inhibitory activity of glucoamylase by electrochemical amperometry. The results show that the baicalin has a good inhibitory activity on glucoamylase with a half maximal inhibitory concentration(IC₅₀) at 0.056 g/L. This study provides a novel method for the extraction and isolation of hypoglycemic components from natural medicines.

Key words: baicalin, glucoamylase, electrochemical method, inhibitory activity

杨剑萍, 苏婷婷, 邱晓珍, 张韩洁, 汪雅丽, 尹伟. 基于电化学安培法的中药降血糖活性成分筛选[J]. 应用化学, 2018, 35(7): 842-848.

Jianping YANG, Tingting SU, Xiaozhen QIU, Hanjie ZHANG, Yali WANG, wei YIN. Screening of Hypoglycemic Constituents in Chinese Herbal Medicine Based on Electrochemical Amperometric Detection[J]. Chinese Journal of Applied Chemistry, 2018, 35(7): 842-848.

参考文献

[1]	Zhu H,Li L,Zhou W,et al. Advances in Non-Enzymatic Glucose Sensors Based on Metal Oxides[J]. J Mater Chem B,2016,4:7333-7349.
[2]	Su T,Xin L,He Y,et al. The Abnormally High Level of Uric D-Ribose for Type-2 Diabetics[J]. Prog Biochem Biophys,2013,40(9):816-825.
[3]	Lee W,Ku S,Bae J. Antiplatelet, Anticoagulant,Profibrinolytic Activities of Baicalin[J]. Arch Pharm Res,2015,38(5):893-903.
[4]	Park K,Lee J S,Choi J S,et al. Identification and Characterization of Baicalin as a Phosphodiesterase 4 Inhibitor[J]. Phytother Res,2016,30(1):144-151.
[5]	Wan L,Jiang X,Liu Y,et al. Screening of Lipase Inhibitors from Scutellaria baicalensis Extract Using Lipase Immobilized on Magnetic Nanoparticles and Study on the Inhibitory Mechanism[J]. Anal Bioanal Chem,2016,408(9):2275-2283.
[6]	Liu X,Zhang W,Chen Z.Preparation of a Novel Molecularly Imprinted Polymer for the Highly Selective Extraction of Baicalin[J]. J Sep Sci,2015,38(24):4233-4239.
[7]	CHEN Lisha,CHANG Fengxia,ZHU Zhiwei.Comparative Study of Copper Compound Based Nonenzymatic Glucose Sensors[J]. J Anal Sci,2014,30(5):677-681(in Chinese). 陈莉莎,常凤霞,朱志伟. 铜基化合物无酶葡萄糖电化学传感器的比较研究[J]. 分析科学学报,2014,30(5):677-681.
[8]	Myung N,Kim S,Lee C,et al. Facile Synthesis of Pt-CuO Nanocomposite Films for Non-enzymatic Glucose Sensor Application[J]. J Electrochem Soc,2016,163(5):B180-B184.
[9]	Yin H,Cui Z,Wang L,et al. In Situ Reduction of the Cu/Cu2O/Carbon Spheres Composite for Enzymaticless Glucose Sensors[J]. Sens Actuators B,2016,222:1018-1023.
[10]	Yang J,Lin Q,Yin W,et al. A Novel Nonenzymatic Glucose Sensor Based on Functionalized PDDA-Graphene/CuO Nanocomposites[J]. Sens Actuators B,2017,253:1087-1095.
[11]	Ohkoshi E,Nagashima T,Sato H,et al. Simple Preparation of Baicalin from Scutellariae Radix[J]. J Chromatogr A,2009,1216(11):2192-2194.
[12]	Luo J,Jiang S,Zhang H,et al. A Novel Non-Enzymatic Glucose Sensor Based on Cu Nanoparticle Modified Graphene Sheets Electrode[J]. Anal Chim Acta,2012,709:47-53.
[13]	Yin M,Liu S.Synthesis of CuO Microstructures with Controlled Shape and Size and Their Exposed Facets Induced Enhanced Ethanol Sensing Performance[J]. Sens Actuators B,2016,227:328-335.
[14]	Zheng J,Zhang W,Lin Z,et al. Microwave Synthesis of 3D Rambutan-like CuO and CuO/Reduced Graphene Oxide Modified Electrodes for Non-enzymatic Glucose Detection[J]. J Mater Chem B,2016,4(7):1247-1253.
[15]	Yu Y,Chen Z,He S,et al. Direct Electron Transfer of Glucose Oxidase and Biosensing for Glucose Based on PDDA-capped Gold Nanoparticle Modified Graphene/Multi-walled Carbon Nanotubes Electrode[J]. Biosen Bioelectron,2014,52:147-152.