核黄素荧光猝灭法测定2,4,6-三硝基苯酚

doi:10.11944/j.issn.1000-0518.2016.05.150367

摘要/Abstract

摘要：

基于核黄素与2,4,6-三硝基苯酚混合后产生荧光猝灭现象,建立了核黄素作为荧光探针测定2,4,6-三硝基苯酚的新方法。在0.2 mol/L磷酸盐(NaH₂PO₄-Na₂HPO₄)缓冲溶液(pH=6.2)中,响应时间为1 min时,检测2,4,6-三硝基苯酚的线性范围为2.5~1000 μmol/L,相关系数为0.9938,检测限为0.55 μmol/L。当加入5.00和20.00 μmol/L 2,4,6-三硝基苯酚到水样后,回收率在98.2%~103.5%之间。方法简便,选择性好,线性范围宽,可用于实际水样中2,4,6-三硝基苯酚的定性定量分析。

关键词: 核黄素, 三硝基苯酚, 荧光猝灭法

Abstract:

A novel method for the determination of 2,4,6-trinitrophenol has been developed based on fluorescent quenching of riboflavin by 2,4,6-trinitrophenol(TNP) in aqueous solutions. The proposed fluorescent quenching method for TNP detection at pH=6.2 using 0.2 mol/L phosphate(NaH₂PO₄-Na₂HPO₄) as buffer solution responses within 1 min and with a broad linear relationship from 2.5 to 1000 μmol/L(r=0.9938). The limit of detection for TNP is 0.55 μmol/L. When 5.00 and 20.00 μmol/L TNP is added to different water samples, the recovery ranges from 98.2% to 103.5%. Furthermore, this method is simple, selective, and with wide linear range. Therefore, it can be applied in the determination of 2,4,6-trinitrophenol in real samples.

Key words: riboflavin, trinitrophenol(TNP), fluorescent quenching method

黄小梅, 邓祥. 核黄素荧光猝灭法测定2,4,6-三硝基苯酚[J]. 应用化学, 2016, 33(5): 606-610.

HUANG Xiaomei, DENG Xiang. Fluorescent Quenching Method for the Detection of 2,4,6-Trinitrophenol Using Riboflavin[J]. Chinese Journal of Applied Chemistry, 2016, 33(5): 606-610.

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