Fenton反应考察抗坏血酸清除羟基自由基能力及动力学

doi:10.11944/j.issn.1000-0518.2015.08.140427

摘要/Abstract

摘要：

自由基的清除对维持正常的生理活动和抗衰老具有重要意义。抗坏血酸(V_C)是常用的天然抗氧化剂之一, 为了有效合理利用V_C的抗氧化性, 实验利用水杨酸捕捉Fenton反应产生的羟基自由基(·OH), 加入V_C考察与水杨酸竞争清除·OH的能力, 选择出最佳清除条件;并根据双底物反应特征, 拟合出Alberty方程, 得到了清除·OH的规律。结果表明, 当V_C用量为4.54 mmol/L、温度为47 ℃条件下、反应90 min时, V_C对·OH的清除率达到最大, 为46.23%;拟合出的反应动力学参数最大反应速率为0.11 mmol/(L·min), 水杨酸和V_C 的特征常数分别为30.2和1.2×10^-3 mmol/L。比较实验结果和拟合方程得到的数据, 二者非常接近, 相对误差在1.66%~5.40% 之间, 从而为合理利用抗坏血酸清除羟基自由基提供理论依据。

关键词: 羟基自由基, 抗坏血酸, 清除率, 反应动力学

Abstract:

For humanbeings, radicals scavenging plays an important role for maintaining the fundamental physiological and anti-ageing need. Comparison for competing scavenging ability of Vitamin C(V_C) with salicylic acid gives optimal scavenging condition for V_C. By fitting the Alberty equation, the maximum 46.23% clearance of ·OH by V_C reached when V_C concentration is 4.54 mmol/L at 47 ℃ for 90 min, and the maximum reaction rate is 0.11 mmol/(L·min). The dissociation constant for salicylic acid and V_C is 30.2 mmol/L and 1.2×10^-3 mmol/L, respectively. The experimental data are well-fitted with relative error of 1.66%~5.40%. This is helpful for the effective and scientific usage of V_C.

Key words: hydroxyl radical, ascorbic acid, clearance rate, reaction kinetics

中图分类号:

李艳, 巩士磊, 车影, 靳利娥, 闫果兰. Fenton反应考察抗坏血酸清除羟基自由基能力及动力学[J]. 应用化学, 2015, 32(8): 948-954.

LI Yan, GONG Shilei, CHE Ying, JIN Li'e, Yan Guolan. Hydroxyl Radical Scavenging Activity and Kinetics of Vitamin C[J]. Chinese Journal of Applied Chemistry, 2015, 32(8): 948-954.

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