中华医学超声杂志

应用化学 ›› 1997, Vol. 0 ›› Issue (2): 28-31.

• 研究论文 • 上一篇    下一篇

水杨醛-5-溴-水杨酰腙与锌的高灵敏度荧光反应及其应用

唐波, 王晓清, 张志德   

  1. 山东师范大学化学系, 济南 250014
  • 收稿日期:1996-09-02 修回日期:1996-11-11 出版日期:1997-04-10 发布日期:1997-04-10
  • 基金资助:
    山东省青年自然科学基金

Highly Sensitive Fluorigenic Reaction of Salicylaldehyde-5-Bromo Salicyloylhydrazone with Zinc and Its Application

Tang Bo, Wang Xiaoqing, Zhang Zhide   

  1. Department of Chemistry, Shandong Normal University, Jinan 250014
  • Received:1996-09-02 Revised:1996-11-11 Published:1997-04-10 Online:1997-04-10

PDF

364

被引次数

46 | 13

摘要： 合成了新荧光试剂水杨醛-5-溴-水杨酰腙(SABSH),详细研究了试剂与锌的荧光反应新体系的最适宜条件。在pH7.9的异丙醇/水(体积比1: 1)溶液中,该试剂与Zn2+形成配合物,组成比为1: 2(金属: 试剂),λex/λem=393nm/465nm.所建立锌的荧光分析方法的线性范围为0～400μg/L,相对标准偏差为1.2%,检出限为1.3μg/L.考察了36种离子的干扰情况。方法成功地用于面粉、大米、人尿和矿泉水中痕量锌的测定。

关键词: 锌, 水杨醛溴水杨酰腙, 荧光分析

Abstract: A new fluorescent reagent, salicylaldehyde 5 bromo salicyloylhydrazone(SABSH), was synthesized and the fluorigenic reaction of this reagent with zinc was studied in detail. Based on this chelation reaction, a spectrofluorimetric method was developed for the determination of zinc in a 2-propanol-water(1: 1, V/V)medium at pH 7 9. Under these conditions the Zn SABSH complex has excitation and emission maxima at 393 nm and 465 nm, respectively. The linear range of the method is from 0 to 400 μg/L, the relative standard deviation of the method is 1.2% and the detection limit is 1.3 μg/L of zinc when a standard addition method is used in the assay. The molar ratio of zinc to reagent is 1: 2. Interferences of thirty six other ions were studied. The method has been successfully applied to the determination of trace zinc in wheat flour, rice, human urine and mineral spring water samples.

Key words: zinc, salicylaldehyde-5-bromo salicyloylhydrazone, spectrofluorimetry

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