应用化学

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CdTe/CdS量子点与丝裂霉素的相互作用及其应用

李萍萍,殷鹏飞,龚会平,刘正清,王晓丹,何佑秋*   

  1. (西南大学化学化工学院 重庆 400715)
  • 收稿日期:2011-07-22 修回日期:2011-08-31 出版日期:2012-06-10 发布日期:2012-06-10
  • 通讯作者: 何佑秋,教授; Tel:023-68367475; Fax:023-68254000; E-mail:heyq@swu.edu.cn; 研究方向:分子光谱分析
  • 基金资助:
    西南大学发光与实时分析重庆市重点实验室资助项目(CSTC,2006CA8006)

A Study on the interaction between CdTe/CdS Quantum Dots with Mitomycin C and its Analytical Application

LI Pingping, YIN Pengfei, GONG Huiping, LIU Zhengqing, WANG Xiaodan, HE Youqiu*   

  1. (School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China)
  • Received:2011-07-22 Revised:2011-08-31 Published:2012-06-10 Online:2012-06-10
  • Contact: Youqiu

摘要: 在水相中合成了硫普罗宁(Tiopronin,TP)修饰的CdTe/CdS量子点(TP-CdTe/CdS QDs)。 利用紫外-可见吸收光谱、荧光光谱研究了TP-CdTe/CdS QDs与丝裂霉素(mitomycin C,MMC)的相互作用机理。 在pH=7.6的tris-HCl缓冲溶液介质中,TP-CdTe/CdS QDs与MMC相互作用,使TP-CdTe/CdS QDs的荧光发生猝灭,并且QDs的荧光强度与MMC的浓度有良好的线性关系(r=0.999 1),线性范围4.7×10-9~1.2×10-8 g/mL,检出限(3σ)为1.4×10-8 g/mL。 此方法快速简便,用于尿样中丝裂霉素的测定,实验结果令人满意。

关键词: CdTe /CdS 量子点, 紫外-可见吸收, 荧光猝灭法, 丝裂霉素

Abstract: Tiopronin(TP) capped with CdTe/CdS quantum dots(TP-CdTe/CdS QDs) was synthesized in aqueous solution. The paper utilized ultraviolet-visible(UV-Vis) absorption spectra and fluorescence spectrometry to study the interactions between TP-CdTe/CdS QDs and mitomycin C(MMC). With the optimum reaction conditions which was found to be at pH=7.6 with tris-HCl buffer solution, the quenched fluorescence intensity of TP-CdTe/CdS QDs was linearly proportional with the concentration of MMC, correlation coefficient r=0.9991, and the detection limits were in the range of 4.7×10-9~1.2×10-8 g/mL, and the detection limit(3σ) for MMC was 1.4×10-8 g/mL. The quantitative determination of MMC is quick, simple and convenient. This method has been applied to the determination of MMC in urine samples with satisfactory results.

Key words: CdTe/CdS QDs, UV-vis, Fluorescence quenching method, Mitomycin C

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