中华医学超声杂志

应用化学 ›› 2010, Vol. 27 ›› Issue (12): 1466-1469.DOI: 10.3724/SP.J.1095.2010.00067

• 研究论文 • 上一篇    下一篇

双抗夹心ELISA法测定水资源、食品及饲料中总甾体激素含量

马德章1,刁昱文2,杨佳新1,王宝德3,于源华1*   

  1. (1.长春理工大学生命科学技术学院 长春 130022;
    2.吉林大学畜牧兽医学院 长春；3.中国石油管道长吉分公司 长春)
  • 收稿日期:2010-01-26 修回日期:2010-05-14 出版日期:2010-12-10 发布日期:2010-12-10
  • 通讯作者: 于源华，教授; E-mail:yuyuanhua8888@126.com; 研究方向：生物分析检测
  • 基金资助:
    吉林省科技厅重大项目（20096012）资助

ELISA detection of Steroidal Hormones in Water Resources, Foods and Feed

MA De-Zhang1, DIAO Yu-Wen2, YANG Jia-Xin1, WANG Bao-De3, YU Yuan-Hua1*   

  1. (1.School of Life Science and Technology,Changchun University of Science and Technology，Changchun 130022;
    2.College of Animal Science and Veterinary Medicine,Jilin University,Changchun;
    3.Changchun Corporation of Chinese Oil Transportation Pipeline,Changchun)
  • Received:2010-01-26 Revised:2010-05-14 Published:2010-12-10 Online:2010-12-10

PDF

1277

被引次数

2

摘要：

基于睾丸酮丛毛单胞菌（C.test）具有降解甾体类物质的特性，其细胞中3α-羟基类固醇脱氢酶/碳酰基还原酶(3α-HSD) 是诱导酶，建立了以3α-HSD的测定总甾体激素含量的双抗夹心酶联免疫反应(ELISA)方法。 实验结果表明，睾丸酮类甾体激素的量在0.5~600 μg/g之间，与3α-HSD酶表达量具有明显线性关系。 应用这种方法对多种水资源、饲料、肉食品进行了检测。 检测结果表明，松花湖水中的睾丸酮类甾体激素含量为34.5 μg/g，明显高于其它水系；被测饲料中睾丸酮类甾体激素含量均大于2 μg/g；肉类样品的检测结果均低于检测下限。 本方法与高效液相色谱法的测定结果相符。

关键词: 甾体激素, 酶联免疫反应, 3α-羟基类固醇脱氢酶/碳酰基还原酶

Abstract:

Comamonas testosteroni secretes 3α-HSD/CR when induced by steroid hormones, then it has the characteristics to degrade steroid hormones. This work developed a Enzyme-linked immunosorbent assay(ELISA) method to detect the steroidal hormones in water, food and animal feed. The results show that the content of testosterone and 3α-HSD enzyme expression have a linear relationship. The linear range is within 0.5~600 μg/L. Adopting this method, the study detect the steroidal hormones in water, food and feed. Content of testosterone in Songhua Lake is 34.5 μg/L, which is higher than others. Content of testosterone in the 8 animal feed is more than 2 μg/L. Meat sample test shows that the amount of steroidal hormones is below the detection limit. This result from this method is consistent with that from HPLC.

Key words: steroid hormones, ELISA, 3α-HSD/CR

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