中华医学超声杂志

应用化学 ›› 2009, Vol. 26 ›› Issue (08): 996-998.

• 研究简报 • 上一篇    下一篇

4种动物药水提液中3种嘌呤碱HPLC法测定

李楠,王琳琳,刘海艳,张洁,刘军,王英华*   

  1. (吉林大学化学学院 长春 130012)
  • 收稿日期:2008-07-07 修回日期:2008-12-28 出版日期:2009-08-10 发布日期:2009-08-10
  • 通讯作者: 王英华，女，教授; E-mail:wangyh@jlu.edu.cn; 研究方向：食品分析、化学计量学技术应用

Determination of Hypoxanthine, Xanthine and Adenine Extracts in Four Animal Drugs by HPLC

LI Nan, WANG Lin-Lin, LIU Hai-Yan, ZHANG Jie, LIU Jun, WANG Ying-Hua*   

  1. (College of Chemistry,Jilin University,Changchun 130012)
  • Received:2008-07-07 Revised:2008-12-28 Published:2009-08-10 Online:2009-08-10

PDF

1439

被引次数

3

摘要：

以甲醇/水为流动相，反相高效液相色谱-紫外检测器测定了乌蛇、蟾蜍、土虫、地龙水提取液中黄嘌呤、次黄嘌呤、腺嘌呤的含量,对样品提取和高效液相色谱条件进行了优化。 黄嘌呤、次黄嘌呤、腺嘌呤的检出限分别为0.012×10-6、0.041×10-6、0.042×10-6  g/mL，样品分析的日内测定相对标准偏差分别小于4.1%、7.3%、4.5%，日间测定相对标准偏差分别小于5.8%、2.9%、3.1%,平均加标回收率为94%～107%。

关键词: 高效液相色谱，超声提取，嘌呤，动物药

Abstract:

Hypoxanthine, xanthine and adenine in some animal drug materials were determined by means of HPLC with methanol/water as the mobile phase and a UV detector. This process of sample preparation was improved and the conditions of HPLC were also optimized. The detection limits of hypoxanthine, xanthine and adenine were 0.012×10-6, 0.041×10-6, 0.042×10-6 g/mL, respectively. The intra-day precisions(RSD) of hypoxanthine, xanthine and adenine were less than 4.1%, 7.3% and 4.5%, respectively. The inter-day precisions(RSD) of hypoxanthine, xanthine and adenine were less than 5.8%, 2.9% and 3.1%, respectively. The recovery rates of the four samples with added specimen were in a range of 94% to 107%.

Key words: high performance liquid chromatography,ultrasound extraction，purine,animal drugs

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