应用化学 ›› 2009, Vol. 26 ›› Issue (12): 1495-1497.

• 研究简报 • 上一篇    下一篇

SPE-GC/MS法检测人血中的鸦片类毒品

王燕燕,孟品佳*   

  1. (中国人民公安大学刑事科学技术系 北京 100038)
  • 收稿日期:2008-11-27 修回日期:2009-05-06 出版日期:2009-12-10 发布日期:2009-12-10
  • 通讯作者: 孟品佳,女,教授; E-mail:mengpinjia@163.com; 研究方向:毒物、毒品分析
  • 基金资助:
    北京教育委员会共建项目建设计划(SYS1004100436)资助

Analysis of Opiates in Human Blood by Solid-Phase Extraction and Gas Chromatography/ Mass Spectrometry Detection

WANG Yan-Yan,MENG Pin-Jia*   

  1. (Department of Forensic Science,Chinese People′s Public Security University,Beijing 100038)
  • Received:2008-11-27 Revised:2009-05-06 Published:2009-12-10 Online:2009-12-10

摘要:

采用混合型强阳离子固相萃取柱(SPE)对血液中的吗啡、可待因、6-单乙酰吗啡3种鸦片类毒品进行提取,提取液吹干,再用N-甲基-双三氟乙酰胺(MBTFA)衍生化,进行GC/MS-SIM检测。以乙基吗啡为内标,3种毒品检测的线性相关系数均大于0.99, 线性范围为10~1000 μg/L,相对回收率分别为90%~116 %、90%~110%、79%~102 % ;日内和日间相对标准偏差分别小于10%和16%;检测限分别为1、0.5和3 μg/L。该方法灵敏度高、重现性好、操作简便,可用于鸦片类毒品滥用者或中毒者血液中的毒品及其代谢物的检测。

关键词: 鸦片类毒品;GC/SIM-MS检测;SPE萃取

Abstract:

Morphine, codeine and 6-acetyl morphine in human blood was detected using solid-phase extraction and gas chromatography/mass spectrometry-selected ion monitoring, with ethyl morphine as internal standard, 3 opiates spiked in blank blood were extracted using a solid-phase cartridge. The fluent was evaporated to dryness followed by being derivatized with MBTFA, then it was analyzed directly by GC/MS in selected ion mode. The method was validated in the range 10~1000ng/mL with the drug-spiked blood, the reactor responded linearly over the studied range (R2>0.99 for the 3 drugs); The relative standard deviations (RSD) for intra and inter-days were less than10% and 16%, respectively the relative extraction recoveries vary from 79% to 116%, and LODs were less than 1, 0.5 and 3ng/mL, which promised the perspective application of the method in detecting trace amount of opium in human blood.

Key words: opiate,GC/MS-SIM detection,solid-phase extraction

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