中华医学超声杂志

应用化学 ›› 2009, Vol. 26 ›› Issue (11): 1357-1361.

• 研究论文 • 上一篇    下一篇

在丙酮增敏作用下硝普钠分光光度法测定盐酸吗啉胍

詹艳艳1,杜新贞1*,李全民2   

  1. (1.西北师范大学化学与化工学院 兰州 730070；2.河南师范大学化学与环境科学学院 新乡)
  • 收稿日期:2008-11-10 修回日期:2009-01-20 出版日期:2009-11-10 发布日期:2009-11-10
  • 通讯作者: 杜新贞，男，教授，博士生导师; E-mail:zhanyanwish@163.com; 研究方向：药物及环境污染物分析
  • 作者简介:

Sodium Nitroprusside Spectrophotometric Determination of Moroxydine Hydrochloride under Sensitization of Acetone

ZHAN Yan-Yan1, DU Xin-Zhen1*, LI Quan-Min2   

  1. (1.College of Chemistry and Chemical Engineering,Northwest Normal University,Lanzhou 730070;
    2.College of Chemistry and Environmental Science,Henan Normal University,Xinxiang)
  • Received:2008-11-10 Revised:2009-01-20 Published:2009-11-10 Online:2009-11-10

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摘要：

摘要：建立了可见分光光度法测定盐酸吗啉胍的新方法。研究表明，当NaOH浓度为0.13mol/L，在丙酮存在下硝普钠与盐酸吗啉胍反应生成紫色N-亚硝基类化合物，其最大吸收波长为508nm，表观摩尔吸光系数2.5×103 L/（mol•cm）。盐酸吗啉胍浓度在0.38×10-6 ~ 3.0×10-4 g/mL范围内与吸光度呈现良好线性关系，线性回归方程A = 0.02292 + 0.01213c (10-6 g/mL)，线性相关系数r=0.9998，检测限(3σ/k)为0.25×10-6 g/mL，RSD = 0.21%。本方法可用于药物样品和血清中盐酸吗啉胍含量的测定。

关键词: 硝普钠, 盐酸吗啉胍, 丙酮, 分光光度

Abstract:

Abstract： A  new method was proposed for the determination of moroxydine hydrochloride with  sodium nitroprusside by spectrophotometry. Colored  N-nitrosamine product was  formed by moroxydine hydrochloride and sodium nitroprusside in alkaline solution. The liner regression equation is A = 0.02292 + 0.01213c (10-6 g/mL) with a correlation coefficient of 0.9998, and ε was 2.5×103 L/(mol•cm) at  508 nm. Beer's law was well obeyed in a range of  moroxydine hydrochloride concentration of 0.38×10-6  ~ 3.0×10-4 g/mL,  the  limit of detection (3σ/k) was 0.25×10-6 g/mL, the RSD was 0.21%. The method was used for  determination of moroxydine hydrochloride in pharmaceutical samples and human serum with satisfactory results.

Key words: sodium nitroprusside, moroxydine hydrochloride, acetone, spectrophotometry

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