荧光法快速测定奶粉中3种雌激素的总量

doi:10.11944/j.issn.1000-0518.2018.05.170210

摘要/Abstract

摘要：

基于3种雌激素(雌二醇、雌三醇和炔雌醇)相似的荧光性质,建立起荧光法直接快速测定奶粉中3种雌激素的总量。以雌三醇为标准参考物质,采用标准曲线法测定。雌三醇的荧光强度与浓度在1~2000 nmol/L的范围内呈线性关系,方法的检出限为0.16 nmol/L,回收率在89.11%~98.49%之间。该方法简单、快速、线性范围宽、准确度和灵敏度高。用于实际奶粉中3种雌激素总量的测定,结果满意。

关键词: 雌激素, 荧光分析法, 奶粉, 雌二醇, 雌三醇, 炔雌醇

Abstract:

Based on the similarity of the fluorescence properties of three estrogens, a new fluorescence method for direct determination of the total amount of three estrogens(estradiol, estriol, ethinyloestradiol) in milk powder was developed. Taking estriol as the standard reference material, the standard curve method was adopted. The fluorescence intensity of estriol has a linear relationship with the concentration in the range of 1.00~2000 nmol/L and the detection limit of 0.16 nmol/L. The recovery is in the range of 89.11%~98.49%. This method is simple, rapid, highly accurate and sensitive and has been successfully applied to determine the total estrogens in milk powder with satisfactory results.

Key words: estrogens, fluorescence spectrometry, milk powder, estradiol, estriol, ethinyloestradiol

蔡其洪, 陈小萍, 鲁家兴, 陈登秀. 荧光法快速测定奶粉中3种雌激素的总量[J]. 应用化学, 2018, 35(5): 609-612.

CAI Qihong, CHEN Xiaoping, LU Jiaxing, CHEN Dengxiu. Rapid Determination of Total Amount of Three Estrogens in Milk Powder by Fluorescence Spectrometry[J]. Chinese Journal of Applied Chemistry, 2018, 35(5): 609-612.

参考文献

[1]	Armstrong B,Doll R.Environmental Factors and Cancer Incidenceandmortality in Different Countries, with Special Reference to Dietary Practices[J]. Int J Cancer,1975,15(4):617-631.
[2]	Rose D P,Boyar A P,Wynder E L.International Comparisons of Mortality Rates for Cancer of the Breast, Ovary, Prostate, and Colon, and Per Capita Food Consumption[J]. Cancer,1986,58(11):2363-2371.
[3]	Qin L Q,Wang P Y,Kaneko T,et al. Estrogen: One of the Risk Factors in Milk for Prostate Cancer[J]. Med Hypoth,2004,62(1):133-142.
[4]	YUAN Lijun,XU Zhuangjian,XU Xiangsheng.The Estrogen in Cow's Milk and Its Safety[J]. Int J Inter Med,2007,34(4):712-714(in Chinese). 袁丽君,徐庄剑,许祥生. 牛奶中的雌激素及其安全性[J]. 国际内科学杂志,2007,34(4):712-714.
[5]	Kaipparettu B A,Malik S,Konduri S D,et al. Estrogenmediated Downregulation of CD24 in Breast Cancer Cells[J]. Int J Cancer,2008,123(1):66-72.
[6]	Wang L,Yan H Y,Yang C L,et al. Synthesis of Mimic Molecularly Imprinted Ordered Mesoporous Silica Adsorbent by Thermally Reversible Semicovalent Approach for Pipette-Tip Solid-Phase Extraction-Liquid Chromatography Fluorescence Determination of Estradiol in Milk[J]. J Chromatogr A,2016,1456(1):58-67.
[7]	Xiao X H,Yin Y,Hu Y L,et al. Microwave-Assisted Extraction Coupled with Single Drop Microextraction and High-Performance Column Liquid Chromatography for the Determination of Trace Estrogen Adulterants in Soybean Isoflavone Dietary Supplements[J]. J AOAC Int,2010,93(3):849-854.
[8]	XIAO Fugang,GUO Weiyun,LIU Yan,et al. Determination of Estradiol in Milk Powder by HPLC[J]. Food Res Dev,2011,32(8):81-83(in Chinese). 肖付刚,郭卫芸,刘燕,等. 奶粉中雌二醇的高效液相色谱法检测[J]. 食品研究与开发,2011,32(8):81-83.
[9]	ZHANG Hongying,ZHANG Hong,WANG Shuhui,et al. Determination of Environmental Estrogens in Milk by Gas Chromatography-Mass Spectrometry[J]. Chinese J Health Lab Technol,2007,17(6):1139-1140(in Chinese). 张红英,张宏,王淑惠,等. 气相色谱-质谱法测定牛奶中的8种环境雌激素和内源性雌激素[J]. 中国卫生检验杂志,2009,19(8):1713-1715.
[10]	LIU Yonglin,TIAN Chengxin,YAN Tianwen,et al. Determination of β-Estradiol in Bovine Colostrum by Gas Chromatography-Mass Spectrometry[J]. China Dairy Ind. ,2014,42(7):53/54/55,59(in Chinese). 刘泳麟,田成新,闫天文,等. 气相色谱-质谱法检测牛初乳中β-雌二醇的研究[J]. 中国乳品工业,2014,42(7)):53/54/55,59.
[11]	Tso J,Aga D S.A Systematic Investigation to Optimize Simultaneous Extraction and liquid Chromatography Tandem Mass Spectrometry Analysis of Estrogens and Their Conjugated Metabolites in Milk[J]. J Chromatogr A,2010,1217(29):4784-4795.
[12]	Yang Y,Shao B,Zhang J,et al. Determination of the Residues of 50 Anabolic Hormones in Muscle, Milk and Liver by Very-High-Pressure Liquid Chromatodraphy-Electrospray Ionization Tandem Mass Spectrometry[J]. J Chromatogr B,2009,877(5/6):489-496.