微流控纺丝制备葡萄糖响应凝胶纤维

doi:10.11944/j.issn.1000-0518.2018.10.170370

摘要/Abstract

摘要：

葡萄糖响应凝胶纤维在血糖监测传感器开发中有着潜在的应用前景。本文基于微流控纺丝方法和自由基聚合反应制备了化学交联和物理交联的葡萄糖响应聚(N-异丙基丙烯酰胺/3-丙烯酰胺基苯硼酸)(P(NIPAM-co-AAPBA))凝胶纤维。通过傅里叶变换红外光谱、扫描电子显微镜和力学性能测试等技术手段表征了凝胶纤维的结构和形态、力学性能、溶胀度动力学与葡萄糖响应性。结果表明,凝胶纤维具有多孔的微观结构,通过改变芯层与壳层溶液的流速可以调控凝胶纤维的直径。随着AAPBA质量分数的增加,凝胶纤维的溶胀速率和平衡溶胀度均有所降低,但葡萄糖响应性能提高。与化学交联的凝胶纤维相比,物理交联的凝胶纤维具有良好的力学性能。

关键词: 微流控纺丝, 凝胶纤维, 葡萄糖响应, 丙烯酰胺基苯硼酸

Abstract:

Glucose responsive gel fibers have potential application prospects in the development of glucose sensors for monitoring blood glucose levels. In this work, poly(N-ispropylacrylamide/3-acrylamidophenylboronic acid)(P(NIPAM-co-AAPBA)) glucose sensitive hydrogel fibers were fabricated via microfluidic spinning and off-chip free radical polymerization reaction. The structure and morphology, mechanical properties, swelling kinetics and glucose responsiveness of the gel fibers were characterized by Fourier transform infrared spectrometer(FTIR), scanning electron microscopy(SEM) and mechanical test. The results show that the gel fibers have porous microstructures, and their diameters could be controlled by changing the flow rates of the core and sheath layer solution. With the increase of AAPBA mass fraction, the swelling rate and equilibrium swelling degree of the gel fibers are decreased, but the glucose response performance is improved. Compared with the chemically cross-linked gel fibers, the physically cross-linked gel fibers have good mechanical properties.

Key words: microfluidic spinning, gel fibers, glucose responsive, acrylamidophenylboronic acid

马园园, 刘晗婷, 彭利, 马敬红, 龚静华. 微流控纺丝制备葡萄糖响应凝胶纤维[J]. 应用化学, 2018, 35(10): 1208-1214.

MA Yuanyuan, LIU Hanting, PENG Li, MA Jinghong, GONG Jinghua. Fabrication of Glucose-Responsive Gel Fibers via Microfluidic Spinning[J]. Chinese Journal of Applied Chemistry, 2018, 35(10): 1208-1214.

参考文献

[1]	MENG Wenwen,MO Jianxun,SUN Wenbo,et al. The Clinical Use of Real-Time Continuous Glucose Monitoring in Hyperglycemic Crises in Adult Patients with Diabetes[J]. World J Tradit Chinese Med,2017,12(A01):47-47(in Chinese). 蒙雯雯,莫建勋,孙文波,等. 实时动态血糖监测在高血糖急性并发症中的应用[J]. 世界中医药,2017,12(A01):47-47.
[2]	GUAN Xiaoyu,HAO Hong,LI Hairui,et al. Research on Glucose-sensitive Hydrogels[J]. Chinese Polym Bull,2009(8):49-53(in Chinese). 管晓玉,郝红,李海瑞,等. 葡萄糖敏感型水凝胶最新研究进展[J]. 高分子通报,2009(8):49-53.
[3]	JIANG Jianxin,LIU Yantao,ZHOU Ziyuan,et al. Research Progress and Applications of Macromolecular Polysaccharide-based Hydrogels for Functional Materials[J]. Chem Ind Forest Prod,2017,37(2):1-10(in Chinese). 蒋建新,刘彦涛,周自圆,等. 高分子多糖水凝胶功能材料研究与应用进展[J]. 林产化学与工业,2017,37(2):1-10.
[4]	ZHANG Yongjun.Glucose-sensitive Polymeric Hydrogels[J]. China Med Device Inf,2009,15(5):18-20(in Chinese). 张拥军. 葡萄糖敏感高分子水凝胶材料[J]. 中国医疗器械信息,2009,15(5):18-20.
[5]	Magee M F,Gourgari E,Youssef G A,et al. mHealth Real-Time Blood Glucose Monitoring Facilitates Glycemic Management[J]. J Diabetes Sci Technol,2017,11(1):172-173.
[6]	Shibata H,Heo Y J,Okitsu T,et al. Injectable Hydrogel Microbeads for Fluorescence-Based in vivo Continuous Glucose Monitoring[J]. Proc Natl Acad Sci USA,2010,107(42):17894-17898.
[7]	Heo Y J,Shibata H,Okitsu T,et al. Long-term in vivo Glucose Monitoring Using Fluorescent Hydrogel Fibers[J]. Proc Natl Acad Sci USA,2011,108(33):13399-13403.
[8]	Jeong W,Kim J,Kim S,et al. Hydrodynamic Microfabricationvia “ On the Fly” Photopolymerization of Microscale Fibers and Tubes[J]. Lab Chip,2004,4(6):576-580.
[9]	Shin S J,Park J Y,Lee J Y,et al. “On the Fly” Continuous Generation of Alginate Fibers Using a Microfludic Device[J]. Langmuir,2007,23(17):9104-9108.
[10]	Lim D,Lee E,Kim H,et al. Multi Stimuli-Responsive Hydrogel Microfibers Containing Magnetite Nanoparticles Prepared Using Microcapillary Devices[J]. Soft Matter,2015,11(8):1606-1613.
[11]	Li S,Davis E N,Anderson J,et al. Development of Boronic Acid Grafted Random Copolymer Sensing Fluid for Continuous Glucose Monitoring[J]. Biomacromolecules,2008,10(1):113-118.
[12]	Peng L,Liu Y,Ma J H,et al. Continuous Fabrication of Multi-Stimuli Responsive Graphene Oxide Composite Hydrogel Fibers by Microfluidics[J]. RSC Adv,2017,7(31):19243-19249.
[13]	Liu W,Xu Z N,Sun L X,et al. Polymerization-induced Phase Separation Fabrication:A Versatile Microfluidic Technique to Prepare Microfibers with Various Cross Sectional Shapes and Structures[J]. Chem Eng J,2017,315:25-34.
[14]	Ma J H,XuY J,Fan B, et al. Preparation and Characterization of Sodium Carboxymethylcellulose/Poly(N-isopropylacrylamide)/Clay Semi-IPN Nanocomposite Hydrogels[J]. Eur Polym J,2007,43(6):2221-2228.