生物纳米孔分析技术研究进展

doi:10.11944/j.issn.1000-0518.2017.08.170103

摘要/Abstract

摘要：

生物纳米孔传感技术因其快速、低成本、无需荧光标记等优点,在化学和生物等诸多研究领域得到广泛应用,已发展成为一种新颖的、独具特色的单分子分析手段。该技术目前主要应用于DNA测序研究,同时在单分子分析领域也取得了令人瞩目的成就。该文简要介绍了生物纳米孔分析技术的原理和生物孔的种类,主要总结了近20年来生物纳米孔在DNA测序和单分子分析中的研究进展并予以了展望。

关键词: 生物纳米孔, DNA测序, 单分子分析

Abstract:

Biological nanopore sensing has been developed as an attractive single-molecule tool with advantages of being rapid, low-cost, and label-free. Nanopores are not only being widely developed for DNA sequencing, but they also have been utilized to analyze a wide range of analytes at the single-molecule level. This review summarizes and prospects the principle of the nanopore analysis and nanpore category, with an emphasis on the progress in nanopore-based DNA sequencing and single-molecule analysis.

Key words: biological nanopore, DNA sequencing, single-molecule analysis

尚积祯,李志,郗冬梅,张书圣. 生物纳米孔分析技术研究进展[J]. 应用化学, 2017, 34(8): 855-867.

SHANG Jizhen,LI Zhi,XI Dongmei,ZHANG Shusheng. Progress in Biological Nanopore-based Analysis Technology[J]. Chinese Journal of Applied Chemistry, 2017, 34(8): 855-867.

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