2-(5-溴-2吡啶偶氮)-5-二乙氨基酚显现人体皮肤表面镀锌工具遗留印迹

doi:10.11944/j.issn.1000-0518.2015.09.150033

摘要/Abstract

摘要：

研究2-(5-溴-2吡啶偶氮)-5-二乙氨基酚(5-Br-PADAP)试剂显现镀锌工具遗留印迹的方法。考察接触时间、时间间隔、试剂浓度、是否洗手等因素对印迹强度及分辨率的影响,并对该方法的可靠性进行验证。实验结果表明,试剂浓度1 g/L、接触时间10 s以上,间隔时间不超过4 h条件下,均能显出较好的印迹。该方法可以灵敏地显现镀锌工具遗留印迹,对非铁金属工具遗留印迹检测进行了很好地补充。

关键词: 法庭科学, (溴-吡啶偶氮)-二乙氨基酚, 镀锌工具遗留印迹, 显现

Abstract:

The detection of imprint left by zinc coatings on human skins was studied. The affecting factors such as contact time, time elapse from the contact to the reaction, concentration of 2-(5-bromo-2-pyridylazo)-5-(diethylamino)phenol(5-Br-PADAP) reagent and washing on the intensity and resolution of imprints were studied and the reliability of this method was verified. As shown in experimental results, good imprints can be obtained with a concentration of 1 g/L of 5-Br-PADAP, contact time of 10 s, time elapse of less than 4 h. This method can develop imprints left by zinc coatings sensitively as a good supplement for the detection of imprints left by nonferrous metal tools.

Key words: forensic science, (bromo-pyridylazo)-(diethylamino)phenol, imprints left by zinc coatings, chromogenic detection

邢卓,杨瑞琴. 2-(5-溴-2吡啶偶氮)-5-二乙氨基酚显现人体皮肤表面镀锌工具遗留印迹[J]. 应用化学, 2015, 32(9): 1093-1098.

XING Zhuo,YANG Ruiqin. Chromogenic Detection of Imprints Left by Zinc Coatings on Human Skins by 2-(5-Bromo-2-pyridylazo)-5-(diethylamino)phenol[J]. Chinese Journal of Applied Chemistry, 2015, 32(9): 1093-1098.

参考文献

[1]	Locard E. L'enquête Criminelle et les Méthodes Scientifiques[M]. E. Flammarion, 1920.
[2]	Stevens J M,Messler H. The Trace Metal Detection Technique(TMDT):A Report Outlining a Procedure for Photographing Results in Color, and Some Factors Influencing the Results in Controlled Laboratory Tests[J]. J Forens Sci,1974,19(3):496-503.
[3]	Almog J,Hirshfeld A,Glattstein B,et al.Chromogenic Reagents for Iron(Ⅱ):Studies in the 1,2,4-Triazine Series[J]. Anal Chim Acta,1996,322(3):203-208.
[4]	Szumera J,Welniak M,Olejniczak A,et al.Transfer of Triazine-iron(Ⅱ) Chromic Complexes Left by Iron Items on Textile Background and Human Skin[J]. J Forens Sci,2010,55(4):944-952.
[5]	WANG Liting,YANG Ruiqin. Comparison and Detection of Iron Traces on Hands with Five Reagents[J]. Chem Reag,2014,36(4):348-352(in Chinese). 王丽婷,杨瑞琴. 5种试剂显现手掌面铁器遗留印迹的比较研究[J]. 化学试剂,2014,36(4):348-352.
[6]	Olejniczak A,Cyganiuk A W,Lukaszewicz J P. 1,2,4-Triazine-Based Chromogenic Reagents for the Detection of Microtraces of Various Metals Left on Human Skin[J]. J Forens Sci,2010,55(3):747-752.
[7]	MA Weixing. The Application Progress of 5-Br-PADAP in Analytical Chemistry[J]. Phys Test Chem Anal Part B:Chem Anal,1994,30(3):180-183(in Chinese). 马卫兴. 5-Br-PADAP在分析化学中的应用进展[J]. 理化检验-化学分册,1994,30(3):180-183.
[8]	WEI Fusheng,ZHU Yurui,SHEN Naikui. A Sensitive Chromatic Reagent:5-Br-PADAP[J]. Chem Reag,1980,(1):52-55(in Chinese). 魏复盛,朱玉瑞,沈乃葵. 高灵敏度显色剂5-Br-PADAP[J]. 化学试剂,1980,(1):52-55.
[9]	WANG Liting,YANG Ruiqin. Detection of Iron Traces on Hands with 1,10-Phenanthroline Hydrate[J]. Chem Reag,2014,36(2):165-168(in Chinese). 王丽婷,杨瑞琴. 手掌面铁器残留物邻菲啰啉显现法的研究[J]. 化学试剂,2014,36(2):165-168.
[10]	JIA Ruyan,HE Yufeng,WANG Rongmin,et al.Advanced in Structure, Extract and Applications of Keratins[J]. Chemistry,2008,(4):265-271(in Chinese). 贾如琰,何玉凤,王荣民,等. 角蛋白的分子构成、提取及应用[J]. 化学通报,2008,(4):265-271.
[11]	Wang C,Li B,Li H X. Zn(Ⅱ) Chelating with Peptides Found in Sesame Protein Hydrolysates:Identification of the Binding Sites of Complexes[J]. Food Chem,2014,(165):594-602.
[12]	Almog J,Bar-Or K L,Leifer A,et al. Detection of Recent Holding of Firearms:Improving the Sensitivity of the PDT Test[J]. Forens Sci Int,2014,(241):55-59.
[13]	Hostynek J J,Hinz R S,Lorence C R,et al.Metals and the Skin, Critical Reviews in Toxicology,23,CRC Press,London,1993,171-235.
[14]	Lansdown A B G. Physiological and Toxicological Changes in the Skin Resulting from the Action and Interaction of Metal Ions[J]. CRC Crit Rev Toxicol,1995,25(5):397-462.
[15]	Almog J,Glattstein B. Detection of Firearms Imprints on Hands of Suspects:Study of the PDT-based Field Test[J]. J Forens Sci,1997,(42):993-996.