一种简易同时蒸馏萃取方法用于八角茴香挥发油提取及其GC-MS分析

doi:10.11944/j.issn.1000-0518.2015.03.140196

摘要/Abstract

摘要：

设计了一种简易同时蒸馏萃取(SSDE)装置并用于八角茴香挥发油提取及其气相色谱-质谱法(GC-MS)分析。该装置由圆底烧瓶、恒压滴液漏斗和回流冷凝管构成。通过对八角茴香挥发油组分的GC-MS分析,比较SSDE法和水蒸汽蒸馏提取法(HD)提取植物源挥发油的效果。结果表明,SSDE法所得挥发油相对含量在0.1%以上的19种化合物占挥发油总量的98.84%(相对标准偏差(RSD)(n=3)均不大于5.59%,其中RSD﹤5%的17种化合物占鉴定组分89.5%。SSDE法和HD法挥发油平均收率分别为7.30%(RSD=2.37%(n=5))和6.67%(RSD=5.26%(n=5))。 SSDE法简便、快速,适用于八角茴香挥发油的提取。

关键词: 同时蒸馏萃取, 水蒸汽蒸馏, 气相色谱/质谱, 挥发油, 八角茴香

Abstract:

A simple simultaneous distillation and solvent extraction (SSDE) method was developed and had been applied for sample preparation of the gas chromatography-mass spectrometry (GC-MS) analysis of volatile components from Illicium Verum. The simple apparatus was composed of a round flask, a constant pressure dropping funnel and a condenser. The constituents of the volatile oil obtained by SSDE were compared with those obtained by hydrodistillation method (HD) followed by GC-MS analysis. In terms of GC-MS analysis of volatile oil, the relative standard deviation (RSD) values(n=3) of measured relative contents(>0.1%) of 19 identified compounds are no more than 5.59%. The total content of above components is 98.84% of the volatile oil, in which there are 17 components in 89.5% content with RSD less than 5%. And the yield of volatile oil obtained by SSDE and HD is 7.30% (RSD 2.37%(n=5)) and 6.67% (RSD 5.26%(n=5)), respectively. These results indicate that SSDE is a simple, suitable method for the extraction of volatile oil from Illicium Verum.

Key words: simultaneous distillation extraction, hydrodistillation, gas chromatography-mass spectrometry, volatile oil, Illicium Verum

中图分类号:

孙玉琼, 夏东海, 董新荣, 李霞. 一种简易同时蒸馏萃取方法用于八角茴香挥发油提取及其GC-MS分析[J]. 应用化学, 2015, 32(3): 356-361.

SUN Yuqiong, XIA Donghai, DONG Xinrong, LI Xia. A Simple Simultaneous Distillation/Solvent Extraction Method Coupled with Gas Chromatography-Mass Spectrometry for the Analysis of Volatile Oils of Illicium Verum[J]. Chinese Journal of Applied Chemistry, 2015, 32(3): 356-361.

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