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微芯片毛细管电泳法快速测定黄花鱼中的碱性嫩黄O

翟海云1*,李江梅1,陈缵光2,周清1,潘育方1   

  1. (1.广东药学院药科学院 广州 510006;2.中山大学药学院 广州 510089)
  • 收稿日期:2012-06-20 修回日期:2012-09-04 出版日期:2013-04-10 发布日期:2013-04-10
  • 通讯作者: 翟海云，副教授; Tel:020-39352135; Fax:020-39352129; E-mail:zhaihaiyun@126.com; 研究方向：药物分析
  • 基金资助:
    国家自然科学基金资助项目(21005021)，广东省科学技术项目(2010B020316010)

Rapid Determination of Auramine O in Yellow croaker by Microchip Capillary Electrophoresis

ZHAI Haiyun1*, LI Jiangmei1, CHEN Zuanguang2, ZHOU Qing1, PAN Yufang1   

  1. (1.Department of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China;
    2.School of Phamaceutical Sciences,Sun Yat-sen University,Guangzhou 510089,China)
  • Received:2012-06-20 Revised:2012-09-04 Published:2013-04-10 Online:2013-04-10
  • Contact: 陈

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1666

被引次数

10 | 1

摘要： 采用微芯片毛细管电泳非接触电导检测法，对黄花鱼中非法添加的工业染料碱性嫩黄O进行了分析。 探讨了缓冲液种类、浓度，分离电压和进样时间等因素对分离检测的影响。 实验选择5.0 mmol/L乳酸缓冲液(pH=3.29)、1.8 kV分离电压，在1.0 min内实现了碱性嫩黄O的快速分离测定。 在优化条件下，碱性嫩黄O浓度的线性范围为5.0～100.0 mg/L，黄花鱼中碱性嫩黄O的检出限为0.2 mg/kg，该法可成功测定黄花鱼中碱性嫩黄O的含量。

关键词: 微芯片毛细管电泳, 非接触电导检测, 碱性嫩黄O

Abstract: A method was developed for the rapid determination of auramine O in yellow croaker by microchip capillary electrophoresis with contactless conductivity detection. Effects of the type and concentration of buffer, separation voltage and injection time on the separation and detection were investigated, and the optimal analysis conditions were obtained in the buffer solution of 5.0 mmol/L lactate(pH=3.29) at the separation voltage of 1.8 kV, where auramine O could be separated and determined within 1.0 min. The linear range of the concentration of auramine O is from 5.0 mg/L to 100.0 mg/L, and the limit of detection is 0.2 mg/kg(S/N=3).The method was used for the analysis of auramine O in the yellow croaker satisfactorily.

Key words: Microchip capillary electrophoresis, Contactless conductivity detection, Auramine O

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