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硝普钠分光光度法测定头孢他啶

孙瑶,吴呈珂,李全民*   

  1. (河南师范大学化学与环境科学学院，河南省环境污染控制重点实验室 新乡 453007)
  • 收稿日期:2011-10-12 修回日期:2011-12-05 出版日期:2012-09-10 发布日期:2012-09-10
  • 通讯作者: 李全民，教授; Tel/Fax:0373-3326336; E-mail:mercury6068@hotmail.com; 研究方向：药物分析
  • 作者简介:
  • 基金资助:
    河南省教育厅自然科学基金资助项目(2008B150011)

Spectrophotometric Determination of Ceftazidime Using Sodium Nitroprusside

SUN Yao, WU Chengke, LI Quanmin*   

  1. (Henan Key Laboratory for Environmental Pollution Control,College of Chemistry and Environmental Science,Henan Normal University,Xinxiang 453007,China)
  • Received:2011-10-12 Revised:2011-12-05 Published:2012-09-10 Online:2012-09-10
  • Contact: li quan-min
  • About author:sun yao，Tel/Fax:0373-3326335；E-mail: sunyaodedipan@163.com; wu cheng-ke ，Tel/Fax:0373-3326335；E-mail: 031186@htu.cn;li quan-min，Tel/Fax:0373-3326336; E-mail: mercury6068@hotmail.com ;

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1475

被引次数

7 | 1

摘要： 建立了硝普钠分光光度法测定头孢他啶的方法。实验结果表明，在碱性条件下，头孢他啶与硝普钠以1∶2的化学计量比反应形成一种红褐色产物，其最大吸收波长为528 nm。 头孢他啶在2.50~810 mg/L浓度范围内与吸光度呈良好线性关系，线性回归方程为A=0.02704+0.00218ρ(mg/L)，线性相关系数r＝0.9987，表观摩尔吸收系数ε528=1.2×103 L/(mol·cm)，检测限(3σ/k)为1.38 mg/L。 本方法操作简便、快速，成功用于药品中头孢他啶含量的测定。

关键词: 硝普钠, 头孢他啶, 分光光度法

Abstract: A new method is proposed to determine ceftazidime using sodium nitroprusside. In an alkaline solution, a brown product is formed by the reaction of ceftazidime with sodium nitroprusside with the stoichiometric ratio of 1∶2. The amount of ceftazidime can be determined owing to the absorbance of the colored product. Absorbency is linear with the concentration of ceftazidime in the range of 2.50~810 mg/L, and the regression equation is A=0.02704+0.00218ρ(mg/L) with a correlation coefficient of 0.9987. The apparent molar absorption coefficient and the detection limit are 1.2×103 L/(mol·cm) and 1.38 mg/L, respectively. This method is simple and fast, the study indicated that the method could be applied to the determination of ceftazidime in injection with satisfactory results.

Key words: sodium nitroprusside, ceftazidime, spectrophotometry

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