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全氟辛烷磺酸与蛋白质体系的共振光散射光谱及其分析应用

吴飞,朱进,谭克俊*   

  1. (三峡库区生态环境教育部重点实验室,西南大学化学化工学院 重庆 400715)
  • 收稿日期:2011-09-02 修回日期:2011-10-14 出版日期:2012-08-10 发布日期:2012-08-10
  • 通讯作者: 谭克俊，副教授; Tel/Fax:023-68367257; E-mail:tankj@swu.edu.cn; 研究方向：环境分析化学
  • 基金资助:
    国家自然科学基金(20877063）和西南大学大型仪器设备开放基金资助项

Resonance Light Scattering Spectra of Perfluorooctane Sulfonate-Protein System and Its Analytical Application

WU Fei, ZHU Jin, TAN Kejun*   

  1. (Key Laboratory of Eco-Environments of Three Gorges Reservoir Region under Ministry of Education of PRC,
    College of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China)
  • Received:2011-09-02 Revised:2011-10-14 Published:2012-08-10 Online:2012-08-10

PDF

881

被引次数

7 | 2

摘要： 研究了牛血清白蛋白(BSA)与全氟辛烷磺酸(PFOS)相互作用的共振光散射(RLS)光谱,建立了PFOS的共振光散射分析方法。 在pH值为4.1的BR缓冲溶液中,全氟辛烷磺酸根阴离子与质子化的BSA通过静电引力和疏水作用形成离子缔合物,引起共振光散射强度(IRLS)显著增强,最大散射波长位于285.0 nm处,增强的散射信号强度与PFOS浓度在0.2～25.0 μmol/L范围内呈线性关系,据此建立了测定PFOS的光散射分析方法,检出限为20.0 nmol/L。 讨论了体系的最佳反应条件及外来物质的干扰,并探讨了反应机理。 建立的共振光散射法用于环境水样中PFOS的测定,RSD≤4.4%。

关键词: 牛血清白蛋白, 全氟辛烷磺酸, 共振光散射

Abstract: The interaction of bovine serum albumin(BSA) with perfluorooctane sulfonate(PFOS) was investigated with resonance light scattering(RLS) spectroscopy. A RLS method for the determination of PFOS was established. In pH=4.1 Britton-Robinson buffer solution, perfluorooctane sulfonate anions reacted with the protonated BSA by electrostatic forces and hydrophobic interactions to form ion-association complexes and resulted in greatly enhanced resonance light scattering signals characterized by a peak at 285.0 nm. The RLS intensity of system was proportional to the content of PFOS in the range of 0.2~25.0 μmol/L, and the limit of detection was 20.0 nmol/L. The optimum reaction conditions and interference of foreign substances were investigated. This RLS method was applied to the determination of PFOS in environmental samples with RSD≤4.4%.

Key words: bovine serum albumin, perfluorooctane sulfonate, resonance light scattering

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