中华医学超声杂志

应用化学 ›› 2010, Vol. 27 ›› Issue (01): 102-106.

• 研究论文 • 上一篇    下一篇

表面分子印迹高分子膜修饰硅胶用于血清中茶碱的固相萃取

李永1,周文辉2,杨黄浩2*,王小如1,2   

  1. (1.中国海洋大学化学化工学院 青岛；2.国家海洋局第一海洋研究所 青岛 266061)
  • 收稿日期:2009-01-15 修回日期:2009-05-08 出版日期:2010-01-10 发布日期:2010-01-10
  • 通讯作者: 杨黄浩，男，研究员; E-mail:hhyang@fio.org.cn; 研究方向：分子印迹及纳米技术
  • 基金资助:
    国家自然科学基金（20775019）国家“八六三”计划（2006AA09Z168）山东省自然科学基金（Y2006B01）国家海洋局青年基金（2007601）资助项目

Preparation of Molecularly Imprinted Polymer Films Modified Silica gel and its Extraction Application

LI Yong1, ZHOU Wen-Hui2, YANG Huang-Hao2*, WANG Xiao-Ru1,2   

  1. (1.College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao;
    2.The First Institute of Oceanography,SOA,Qingdao 266061)
  • Received:2009-01-15 Revised:2009-05-08 Published:2010-01-10 Online:2010-01-10

PDF

1474

被引次数

55 | 5

摘要：

 采用表面引发可逆加成-断裂链转移自由基聚合反应，在硅胶表面修饰了分子印迹高分子膜(MIP-silica)。以元素分析和氮吸附分析对修饰的分子印迹高分子膜进行了表征。与传统采用本体聚合合成的分子印迹高分子相比，MIP-silica具有更好的传质能力。本文合成的茶碱印迹MIP-silica可以作为选择性固相萃取材料从血清中富集、检测微量的茶碱，该法合成的MIP-silica还可用于高效液相色谱和毛细管电色谱等领域。

关键词: 分子印迹高分子, 可逆加成－断裂链转移自由基聚合, 硅胶, 茶碱

Abstract:

This paper describes the use of surface initiated reversible addition-fragmentation chain transfer (RAFT) polymerization to graft thin molecularly imprinted polymer (MIP) films from the silica gel particles (MIP-Silica). The grafted imprinted polymer film was characterized by elemental analysis and nitrogen adsorption analysis. The MIP-silica prepared in this study had improved mass-transfer properties compared with MIPs prepared by conventional bulk polymerization. In addition, this paper also proved that the MIP-Silica can be used as the sorbent in solid-phase extraction to determine theophylline in blood serum with satisfactory recovery. Furthermore, the MIP-silica can also be applied in HPLC and capillary electrochromatography.

Key words: molecularly imprinted polymer, Reversible Addition-Fragmentation chain Transfer, silica gel, theophylline

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