中华医学超声杂志

应用化学 ›› 2009, Vol. 26 ›› Issue (08): 981-984.

• 研究论文 • 上一篇    下一篇

醇脱氢酶在反胶束溶液中的催化动力学性质

何进星,柳畅先*,孙小梅   

  1. (中南民族大学化学与材料科学学院,分析化学国家民委重点实验室 武汉 430074)
  • 收稿日期:2008-08-04 修回日期:2008-10-09 出版日期:2009-08-10 发布日期:2009-08-10
  • 通讯作者: 柳畅先，女，教授; E-mail:changxianliu@sina.com; 研究方向：生化分析
  • 基金资助:
    中南民族大学自然科学基金资助项目(YZZ05018)

Enzymatic Kinetics Characterizations of the Immobilized Alcohol Dehydrogenase in Reversed Micelles

HE Jin-Xing, LIU Chang-Xian*, SUN Xiao-Mei   

  1. (Key Laboratory for Analytical Chemistry of the State Ethnic Affairs Commission,College of
    Chemistry and Material Science,South-Central University for Nationalities,Wuhan 430074)
  • Received:2008-08-04 Revised:2008-10-09 Published:2009-08-10 Online:2009-08-10

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2155

被引次数

4 | 1

摘要：

以十六烷基三甲基溴化铵(CATB)-辛烷-己醇反胶束体系对醇脱氢酶(ADH)进行固定化，试验了含水量、酶液pH值、CTAB和己醇浓度对ADH固定化的影响。对游离酶和固定化酶的催化动力学性质研究表明：酶促反应的最适pH值分别为8.2和8.8，最适温度分别是31℃和20℃，对乙醇的米氏常数Km分别为12mmol/L和7.4mmol/L。在30℃时，游离酶存放150min后失活90%，固定化酶失活50%，表明反胶束固定化ADH有较好的热稳定性。

关键词: 反胶束, 醇脱氢酶, 固定化, 酶催化动力学, 抑制作用

Abstract:

The immobilization for alcohol dehydrogenase(ADH) with cetyl trimethyl ammonium bromide(CTAB)-octane-hexanol reversed micelles system was introduced.The effect for the immobilization of the w0(=[water]/[CTAB]),the pH value in enzyme solution,the concentration of CTAB and hexanol were assayed.The study on the kinetic characterizations of the free and immobilized enzyme indicates that optimum pH value of the enzymatic reaction were 8.2 and 8.8, the optimum temperature were 31℃ and 20℃ respectively. The Km value for alcohol were 12mmol/L and 7.4mmol/L respectively. The enzymes were deposited for 150min at 30℃, the activities were lost 90% and 50% for the free enzyme and the immobilized enzyme respectively. It indicates that the immobilized ADH in reversed micelles has better thermal stability.

Key words: reversed micelles, alcohol dehydrogenase, immobilization, enzymatic kinetics, inhibition

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