中华医学超声杂志

应用化学 ›› 1994, Vol. 0 ›› Issue (5): 1-4.

• 研究论文 •    下一篇

磁性高分子微球固定化中性蛋白酶的研究

李孝红, 章贤明, 丁小斌, 孙宗华   

  1. 中国科学院成都有机化学研究所 成都 610041
  • 收稿日期:1993-12-06 修回日期:1994-03-23 出版日期:1994-10-10 发布日期:1994-10-10
  • 基金资助:
    国家自然科学基金资助项目

Immobolization of Neutral Protease onto Magnetic Polymer Microspheres

Li Xiaohong, Zhang Xianming, Ding Xiaobing, Sun Zonghua   

  1. Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041
  • Received:1993-12-06 Revised:1994-03-23 Published:1994-10-10 Online:1994-10-10

PDF

404

被引次数

21 | 6

摘要： 以表面带羟基的磁性高分子微球为载体,对位苯醌活化后,通过共价结合修饰中性蛋白酶,得到比活性为1000U/g的磁性固定化酶。偶联蛋白量20～30mg/g载体,固定化酶活性保持达40%,自由酶和固定化酶相比,最适温度从50℃变到50～60℃,最适pH从7.5变到6.5,Km从0.054%变到0.088%酪蛋白溶液,pH稳定性、热稳定性、贮存稳定性都有较大提高。

关键词: 磁性高分子微球, 中性蛋白酶, 固定化

Abstract: A neutral protease from Balilius Subtilis has been immobilized onto the magnetic polymer microspheres,which were obtained from styrene,hydroxyethyl methacrylate,divinyl benzene and Fe3O4,and had hydroxyl groups in the surface by covalent coupling.The magnetic immobilized neutral protease had a enzyme activity of 1000U/g,enzyme yield of 20～30mg/g carrier,and activity retention of about 40%.For both free enzyme and immobilized enzyme the optimum work temperatures were 50℃ and 50～60℃,the optimum pH values were 7.5 and 6.5, and the kinetic constants were 0.054% and 0.088%,respectively.The thermal stability, pH stability,and the storage stability of the immobilized enzyme are improved obviously.

Key words: magnetic polymer microsphere, neutral protease, immobilization

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