中华医学超声杂志

应用化学 ›› 1993, Vol. 0 ›› Issue (2): 9-12.

• 研究论文 • 上一篇    下一篇

氨化大孔球状聚氯乙烯固定化木瓜蛋白酶的研究

陶国良, 李彦锋, 卓仁禧   

  1. 武汉大学化学系 430072 武汉
  • 收稿日期:1992-04-07 修回日期:1992-07-10 出版日期:1993-04-10 发布日期:1993-04-10

IMMOBILIZATION OF PAPAIN ON AMINATED MACROPOROUS PVC BEAD

Tao Guoliang, Li Yanfeng, Zhuo Renxi   

  1. Department of Chemistry, Wuhan University, 430072 Wuhan
  • Received:1992-04-07 Revised:1992-07-10 Published:1993-04-10 Online:1993-04-10

PDF

376

被引次数

37 | 8

摘要： 以氨化大孔球状聚氯乙烯为载体,采用戊二醛载体交联的方法,将木瓜蛋白酶进行了固定化。以酪蛋白为底物,测定了固定化酶的活力回收。研究了固定化条件对固定化酶活力回收的影响。同时,对所得固定化酶的性质,如温度-活力关系、pH-活力关系、热稳定性以及重复使用性进行了考察。结果表明,所得固定化木瓜蛋白酶具有较好的稳定性和重复使用性。

关键词: 木瓜蛋白酶, 氨化聚氯乙烯, 固定化酶

Abstract: Aminated macroporous PVC bead was used as carrier to prepare immobilized papain samples by glutaraldehyde through crosslinking reaction. The hydrolytic activity of the immobilized papain was determined using casein as substrate. The factors affecting the activity of immobilized papains such as reaction tirne,temperature,pH,amount of papaln and concentration of glutaraldehyde were studied. The immobilized papain has a remarkable increase in the stability and better reuse ability than natural papain.

Key words: papain, aminated PVC, immobilized enzyme

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